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首页> 外文会议>Induced mutations and molecular techniques for crop improvement >ABUNDANCE AND DISTRIBUTION OF SIMPLE SEQUENCE REPEATS IN THE MUSACEAE FAMILY: MICROSATELLITE MARKERS TO MAP THE BANANA GENOME
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ABUNDANCE AND DISTRIBUTION OF SIMPLE SEQUENCE REPEATS IN THE MUSACEAE FAMILY: MICROSATELLITE MARKERS TO MAP THE BANANA GENOME

机译：穆萨科家族中简单序列重复的丰度和分布：微卫星标记以定位香蕉基因组

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The usefulness of microsatellite derived markers is emphasized by implementing relatively simple laboratory techniques (polymerase chain reaction (PCR) and non-radioactive detection) compared with restriction fragment length polymorphism (RFLP) markers. Development of microsatellite markers is currently limited in plants because of the lack of available published sequence data for most species. This applies particularly to tropical crops such as the banana, but an increasing number of research groups are working on molecular characterization of these crops and there is no doubt that more DNA sequences will be available in the near future. A partial, size fractioned PstI genomic library for Musa, previously established as a source for RFLP makers, has been rescreened for dinucleotide repeats. Preliminary results are given of the dinucleotide microsatellite sequences in the banana genome. Roughly one-sixth (17%) of the clones of the banana library contain at least one microsatellite site, as confirmed by sequencing. Surprisingly, the majority of dinucleotide repeats found are of the (GA)_n sequence family. These recently developed microsatellite markers on the banana proved to be abundant and polymorphic (the number of alleles per locus varying between 11 and 24 in a testing panel of 19 accessions representing the different sections of the Musaceae family). Laboratory protocols have been streamlined and a previ-ously described high resolution, non-radioactive analysis procedure (based on denaturing silver stained polyacrylamide gel electrophoresis) has been adapted to detect the variable number of tandem repeat alleles in order to promote exportation of microsatellite markers (i.e. PCR assay kits containing extensively tested, robust, primer pairs) to developing countries. Preliminary results show the efficiency of polymorphic microsatellite markers for mapping and clonal identification using rapid and simple methods. All the microsatellites that have been detected in the banana to date are conserved in sections of the Musaceae family.
机译：与限制性片段长度多态性（RFLP）标记相比，通过实施相对简单的实验室技术（聚合酶链反应（PCR）和非放射性检测）强调了微卫星衍生标记的实用性。由于缺乏大多数物种可用的公开序列数据，目前植物中微卫星标记的开发受到限制。这尤其适用于热带作物，例如香蕉，但是越来越多的研究小组正在研究这些作物的分子特性，毫无疑问，不久的将来将有更多的DNA序列可用。已重新筛选了Musa的部分大小分级的PstI基因组文库，该文库先前已建立为RFLP制造商的来源，现已重新筛选了二核苷酸重复序列。给出了香蕉基因组中二核苷酸微卫星序列的初步结果。测序证实，香蕉文库的大约六分之一（17％）的克隆至少包含一个微卫星位点。令人惊讶地，发现的大多数二核苷酸重复序列是（GA）_n序列家族。这些最近在香蕉上开发的微卫星标记被证明是丰富的和多态的（在代表Musaceae家族不同部分的19个种质的测试小组中，每个基因座的等位基因数量在11和24之间变化）。简化了实验室规程，并且先前描述的高分辨率，非放射性分析程序（基于变性的银染聚丙烯酰胺凝胶电泳）已被用于检测可变数目的串联重复等位基因，以促进微卫星标记的输出（即PCR检测试剂盒，其中包含对发展中国家进行了广泛测试的可靠的引物对。初步结果表明，使用快速简便的方法，多态性微卫星标记可用于作图和克隆鉴定。迄今为止，在香蕉科中发现的所有微卫星都保存在Mus科中。

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  • 会议地点 Vienna(AT)
  • 作者

    P.J.L. LAGODA; J.-L. NOYER; D. DAMBIER; F.-C. BAURENS; C. LANAUD;

  • 作者单位

    Laboratoire d'analyse des genomes des especes tropicales, Unite de recherche des biotechnologies appliquees a 1'amelioration des especes tropicales, Departement de gestion, recherche, documentation et appui technique Centre de cooperation internationale en recherche agronomique pour le deVeloppement (CIRAD), Montpellier, France;

    Laboratoire d'analyse des genomes des especes tropicales, Unite de recherche des biotechnologies appliquees a 1'amelioration des especes tropicales, Departement de gestion, recherche, documentation et appui technique Centre de cooperation internationale en recherche agronomique pour le deVeloppement (CIRAD), Montpellier, France;

    Departement fruits, legumes et horticulture Centre de cooperation internationale en recherche agronomique pour le deVeloppement (CIRAD), Mon;

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  • 正文语种 eng
  • 中图分类 作物遗传育种与良种繁育;
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