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Length polymorphisms in microsatellite (simple sequence repeat - SSR) DNA markers and their use as genetic markers in pine
Length polymorphisms in microsatellite (simple sequence repeat - SSR) DNA markers and their use as genetic markers in pine
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机译：微卫星（简单重复序列-SSR）DNA标记的长度多态性及其在松树中的遗传标记
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摘要
Microsatellite, simple sequence repeat (SSR), markers have much potential for enhancing genome mapping and genotype identification research in forest genetics and tree breeding. SSR markers were developed by isolating and sequencing 1539 loblolly pine SSR clones for 11 SSR motifs. After screening out redundancy among the sequences, 566 oligonucleotide PCR primer pairs flanking the SSRs were synthesised and evaluated for their ability to amplify genomic DNA from loblolly pine. The three SSR motifs that yielded the highest proportion of informative markers from sequenced clones were (AC)n, (AAAT)n, and (AAAC)n. Eighteen polymorphic tri- and tetranucleotide SSR loci were genotyped in 20 loblolly pine trees using automated fluorescent marker analysis. The average number of alleles per locus observed was 6.4, and the average polymorphism information content (PIC) was 0.547. Subsets of the 566 primer pairs were evaluated for their ability to amplify DNA from six other pine species, and 54 primer pairs amplified markers that were polymorphic among the species. Also described are the methods of using the identified SSR loci as genetic markers.
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