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首页> 外文会议>Information technology and agricultural engineering >Antifungal Activity of Extracts by Supercritical Carbon Dioxide Extraction from Roots of Stellera chamaejasme L. and Analysis of Their Constituents Using GC-MS
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Antifungal Activity of Extracts by Supercritical Carbon Dioxide Extraction from Roots of Stellera chamaejasme L. and Analysis of Their Constituents Using GC-MS

机译：超临界二氧化碳萃取虎头草根的提取物的抗真菌活性及其成分的GC-MS分析

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摘要

In this work the supercritical fluid extraction with carbon dioxide was applied to obtain the extracts from roots of Stellera chamaejasme L. The extracts were evaluated through the antifungal activity against Monilinia fructicola. Furthermore, the active compounds from the extracts were separated by macro porous resin and analyzed using gas chromatography-mass spectrometry (GC-MS). The results showing that the extracts, obtained using supercritical carbon dioxide (SC-CO_2) extraction technique, had strong antifungal activity against M. fructicola with inhibition ratio of 88.71% at 2000 μg/mL, minimum inhibitory concentration (MIC) of 250 ug/mL, and minimum fungicidal concentration (MFC) of 2000 μg/mL. After separation with macro porous resin chromatography, fourteen fractions were collected from the SC-CO_2 extracts. At concentration of 2000 μg/mL, fraction 4, 7, and 12 presented highly antifungal activity against M. fructicola, with inhibition ratio of 83.97 %, 80.23 %, and 75.69 %, respectively. Followed by the GC-MS analysis, the main active compounds from fraction 4, 7, and 12 included hexanedioic acid, bis (2-ethylhexyl) ester, πsitosterol, 7-methyl-Z-tetradecen-1-ol acetate9, 9-hexadecenoic acid-hexadecyl ester (Z), 1, 2-benzenedicarboxylic acid-diisooctyl ester, (3π24Z) stigmasta-5, 24(28)-dien-3-ol, stigmastan-3, 5-diene, and squalene.
机译：在这项工作中，采用二氧化碳超临界流体萃取从狼尾草的根中提取提取物。提取物通过对褐飞虱的抗真菌活性进行评估。此外，提取物中的活性化合物用大孔树脂分离，并使用气相色谱-质谱（GC-MS）分析。结果表明，采用超临界二氧化碳（SC-CO_2）提取技术获得的提取物具有较强的抗果蝇抗真菌活性，在2000μg/ mL时抑制率为88.71％，最小抑菌浓度（MIC）为250 ug / mL。 mL，最小杀真菌浓度（MFC）为2000μg/ mL。用宏观多孔树脂色谱分离后，从SC-CO_2提取物中收集了十四个馏分。在浓度为2000μg/ mL时，级分4、7和12表现出对果蝇的高度抗真菌活性，抑制率分别为83.97％，80.23％和75.69％。然后进行GC-MS分析，馏分4、7和12中的主要活性化合物包括己二酸，双（2-乙基己基）酯，谷甾醇，7-甲基-Z-十四烯-1-醇乙酸酯9、9-十六烯酸酸-十六烷基酯（Z），1，2-苯二甲酸-二异辛酯，（3π24Z）stigmasta-5、24（28）-dien-3-ol，stigmastan-3、5-二烯和角鲨烯。

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  • 来源
  • 会议地点 Sanya(CN);Sanya(CN)
  • 作者

    Xue-na Bai; Jun Cheng; Wei Liang; Lan-qing Ma; Yu-bo Liu; Guang-lu Shi; You-nian Wang;

  • 作者单位

    Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. China Beijing University of Agriculture, Beijing, China;

    Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. China Beijing University of Agriculture, Beijing, China;

    Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. China Beijing University of Agriculture, Beijing, China;

    Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. China Beijing University of Agriculture, Beijing, China;

    Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. China Beijing University of Agriculture, Beijing, China;

    Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. China Beijing University of Agriculture, Beijing, China;

    Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. China Beijing University of Agriculture, Beijing, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 信息处理（信息加工）;
  • 关键词

    antifungal activity; GC-MS; SC-CO_2 extraction; stellera chamaejasme L;

    机译：抗真菌活性气相色谱-质谱SC-CO_2萃取；狼毒;

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