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首页> 外文会议>Instrumentation Science and Technology Vol.2 >Design of Integrated Instrument for Polymerase Chain Reaction, Biochip Hybridization and Fluorescence Detection Part Two: A New Biochip to Detection Hepatitis B Virus Mutation
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Design of Integrated Instrument for Polymerase Chain Reaction, Biochip Hybridization and Fluorescence Detection Part Two: A New Biochip to Detection Hepatitis B Virus Mutation

机译：聚合酶链反应，生物芯片杂交和荧光检测集成仪器的设计第二部分：检测乙型肝炎病毒突变的新型生物芯片

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The PHFT machine is capable of performing polymer chain reaction, genechip hybridization and fluorescence detection automatically. There are fourteen tubes in the new biochip and the temperature in each tube can be changed quickly. Every tube contains a glass tube, a cell and a piece of glass. A aminosilane was used to modify glass surface and it was successfully used for attaching prefabricated DNA of oligonucleotides. The experiment showed that the PCR hybridization chip could distinguish an internal single base mismatch in 20mer oligonucleotides. The probes designed to detect the hepatitis B virus and some of its mutations were immobilized on a glass. The genome of hepatitis B virus in blood is separated and amplified and fluorescence labeled. After labeling, PCR products were hybridized to those probes; the PHFT instrument could detect the fluorescence image and calculate the fluorescence intensity. The results indicate the new biochip will find application in clinical HBV and its mutation detection.
机译：PHFT机器能够自动执行聚合物链反应，基因芯片杂交和荧光检测。新的生物芯片中有14个试管，每个试管中的温度可以快速更改。每个试管包含一个玻璃管，一个电池和一块玻璃。氨基硅烷用于修饰玻璃表面，并成功用于附着寡核苷酸的预制DNA。实验表明，PCR杂交芯片可以区分20mer寡核苷酸内部的单碱基错配。用于检测乙型肝炎病毒及其某些突变的探针被固定在玻璃杯上。分离并扩增血液中乙型肝炎病毒的基因组并进行荧光标记。标记后，将PCR产物与这些探针杂交。 PHFT仪器可以检测荧光图像并计算荧光强度。结果表明，新的生物芯片将在临床乙肝病毒及其突变检测中找到应用。

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