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首页> 外文会议>International Symposium on Instrumentation Science and Technology >Design of Integrated Instrument for Polymerase Chain Reaction, Biochip Hybridization and Fluorescence Detection Part Two: A New Biochip to Detection Hepatitis B Virus Mutation
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Design of Integrated Instrument for Polymerase Chain Reaction, Biochip Hybridization and Fluorescence Detection Part Two: A New Biochip to Detection Hepatitis B Virus Mutation

机译：聚合酶链反应综合仪设计，生物芯片杂交和荧光检测部分：一种新的Biochip检测乙型肝炎病毒突变

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The PHFT machine is capable of performing polymer chain reaction, genechip hybridization and fluorescence detection automatically. There are fourteen tubes in the new biochip and the temperature in each tube can be changed quickly. Every tube contains a glass tube, a cell and a piece of glass. A aminosilane was used to modify glass surface and it was successfully used for attaching prefabricated DNA of oligonucleotides. The experiment showed that the PCR hybridization chip could distinguish an internal single base mismatch in 20mer oligonucleotides. The probes designed to detect the hepatitis B virus and some of its mutations were immobilized on a glass. The genome of hepatitis B virus in blood is separated and amplified and fluorescence labeled. After labeling, PCR products were hybridized to those probes; the PHFT instrument could detect the fluorescence image and calculate the fluorescence intensity. The results indicate the new biochip will find application in clinical HBV and its mutation detection.
机译：PHFT机器能够自动进行聚合物链反应，GeneChip杂交和荧光检测。新的生物芯片中有十四管，可以快速改变每个管中的温度。每个管含有玻璃管，电池和一块玻璃。使用氨基硅烷改性玻璃表面，并且成功地用于附着寡核苷酸的预制DNA。实验表明，PCR杂交芯片可以区分20mer寡核苷酸中的内部单碱基错配。设计用于检测乙型肝炎病毒的探针和其一些突变在玻璃上固定在玻璃上。分离和扩增乙型肝炎病毒的乙型肝炎病毒的基因组，并标记荧光。标记后，PCR产物与这些探针杂交; PHFT仪器可以检测荧光图像并计算荧光强度。结果表明，新的Biochip将在临床HBV中发现应用及其突变检测。

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