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Technologically important extremophile 16S rRNA sequence Shannon entropy and fractal property comparison with long term dormant microbes

机译:具有重要技术意义的极端微生物16S rRNA序列Shannon熵和长期休眠微生物的分形特性比较

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Technologically important extremophiles including oil eating microbes, uranium and rocket fuel perchlorate reduction microbes, electron producing microbes and electrode electrons feeding microbes were compared in terms of their 16S rRNA sequences, a standard targeted sequence in comparative phylogeny studies. Microbes that were reported to have survived a prolonged dormant duration were also studied. Examples included the recently discovered microbe that survives after 34,000 years in a salty environment while feeding off organic compounds from other trapped dead microbes. Shannon entropy of the 16S rRNA nucleotide composition and fractal dimension of the nucleotide sequence in terms of its atomic number fluctuation analyses suggest a selected range for these extremophiles as compared to other microbes; consistent with the experience of relatively mild evolutionary pressure. However, most of the microbes that have been reported to survive in prolonged dormant duration carry sequences with fractal dimension between 1.995 and 2.005 (N = 10 out of 13). Similar results are observed for halophiles, red-shifted chlorophyll and radiation resistant microbes. The results suggest that prolonged dormant duration, in analogous to high salty or radiation environment, would select high fractal 16S rRNA sequences. Path analysis in structural equation modeling supports a causal relation between entropy and fractal dimension for the studied 16S rRNA sequences (N = 7). Candidate choices for high fractal 16S rRNA microbes could offer protection for prolonged spaceflights. BioBrick gene network manipulation could include extremophile 16S rRNA sequences in synthetic biology and shed more light on exobiology and future colonization in shielded spaceflights. Whether the high fractal 16S rRNA sequences contain an asteroid-like extra-terrestrial source could be speculative but interesting.
机译:对具有技术重要性的极端微生物(包括食用油的微生物,铀和火箭燃料的高氯酸盐还原微生物,产生电子的微生物和给电子的电极送入的微生物)的16S rRNA序列进行了比较,这是比较系统发生研究中的标准靶向序列。还研究了据报道存活较长时间的微生物。例子包括最近发现的微生物,该微生物在咸水环境中生存了34,000年,同时从其他捕获的死微生物中摄取了有机化合物。就其原子序数波动分析而言,16S rRNA核苷酸组成的香农熵和核苷酸序列的分形维数表明,与其他微生物相比,这些极端微生物的选定范围;与相对温和的进化压力的经验相一致。然而,据报道大多数在延长的休眠期中存活的微生物携带的分形维数介于1.995和2.005之间的序列(N = 13中的10)。对于嗜盐菌,红移叶绿素和抗辐射微生物观察到相似的结果。结果表明,与高盐或辐射环境类似,延长休眠时间将选择高分形的16S rRNA序列。结构方程模型中的路径分析支持所研究的16S rRNA序列(N = 7)的熵和分形维数之间的因果关系。高分形16S rRNA微生物的候选选择可以为长时间的飞行提供保护。 BioBrick基因网络操作可能包括合成生物学中的极端微生物16S rRNA序列,并进一步揭示了外生物学和未来在屏蔽航天中的定殖。高分形的16S rRNA序列是否包含小行星样的地球外来源可能是推测性的,但很有趣。

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