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Comparison of conserved structural and regulatory domains within divergent 16S rRNA–23S rRNA spacer sequences of cyanobacteria

机译：分发16S rRNA-23S rRNA-23s rRNA间隔序列中的保守结构和调节结构域的比较

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PCR amplification of the internal transcribed spacer (ITS) between the 16S rRNA and 23S rRNA genes of the cyanobacterium Nostoc PCC 7120 gave three products. Two represented true ITS regions of different sizes, while the third was a heteroduplex. The longer spacer (ITS-L) contained 512 nucleotides and carried tRNAIle and tRNAAla genes, separated by a large stem–loop structure (V2) composed of short tandemly repeated repetitive sequences. Both tRNA genes, and the 5′ half of the intervening stem, were absent from the shorter spacer (ITS-S), of length 283 nucleotides, which was otherwise almost completely identical to ITS-L. The two spacer regions of Nostoc PCC 7120 were aligned to published ITS sequences of cyanobacteria, the cyanelle of Cyanophora paradoxa and Escherichia coli. Although the ITS regions of cyanobacteria vary in length from 283 to 545 nucleotides and contain either both tRNAIle and tRNAAla genes, only the tRNAIle gene, or neither, there is no correlation between ITS size and coding capacity for tRNAs. Putative secondary structures were determined for the deduced transcripts of the rrn operons of several cyanobacteria and were compared to that of E. coli. Highly conserved motifs important for folding and for maturation of the rRNA transcripts were identified, and regions homologous to bacterial antiterminators (box B–box A) were located. The conserved and variable regions of the cyanobacterial ITS are potential targets of PCR primers and oligonucleotide probes for detection and identification of cyanobacteria at different taxonomic levels.
机译：在16S rRNA和23S rRNA基因之间的内部转录间隔物（其）的PCR扩增是NoStoc PCC 7120的三种产品。两个代表了其不同尺寸的真实区域，而第三则是异单元。较长的间隔物（IT-1）含有512个核苷酸和携带的TRNAILE和TRNAALA基因，由由短串联重复的重复序列组成的大茎环结构（V2）分离。在较短的间隔件（ITS-S）中，不存在长度为283个核苷酸的TRNA基因和5'一半，其几乎完全相同于IT-L. Nostoc PCC 7120的两个间​​隔区被对齐至发表其序列，Cyanophora悖论和大肠杆菌Cyanelle。虽然其区域的细胞区域的长度从283〜545个核苷酸变化，并且含有Trnaile和三环基因，只有Trnaile基因，或者既不是，其尺寸与TRNA的编码能力之间没有相关性。测定的二次结构针对几个蓝菌的RRN操纵子的推导转录物测定，并与大肠杆菌的RRN操纵量进行了比较。鉴定了对折叠和rRNA转录物的折叠成熟的高度保守的图案，并且对细菌抗钻剂（盒子B盒A）同源的区域。蓝藻的保守和可变区是PCR引物和寡核苷酸探针的潜在靶标，用于在不同的分类水平下的蓝藻进行检测和鉴定Cyanobacteria。

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