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首页> 外文会议>International Biohydrometallurgy Symposium, IBS-2001 Sep 16-19, 2001, Ouro Preto, Minas Gerais, Brazil >Isolation and preliminary identification of some iron- and sulfur-oxidizing microorganisms from the Sarcheshmeh copper mine
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Isolation and preliminary identification of some iron- and sulfur-oxidizing microorganisms from the Sarcheshmeh copper mine

机译：Sarcheshmeh铜矿中一些铁和硫氧化微生物的分离和初步鉴定

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摘要

Isolation, partial identification and copper tolerance of some native species of the three major groups of iron and/or sulfur oxidizing prokaryotes (mesophiles, moderate and extreme thermophiles) from the Sarcheshmeh Copper Mine are described. There are vast reserves of low grade copper ores (about 280,000,000 tones) in this mine which the NICICo (National Iranian Copper Industries Company) plans to bioleach it, therefore it was considered to elucidate the Mine's native microflora, and then to determine their tolerance of leaching conditions, such as low pH values, high metal concentrations and pulp densities. Samples were taken from certain parts of the mine and cultured in defined liquid media. Representative strains of all three temperature-related groups used in bioleaching were isolated from which some purified cultures were obtained. These were a strain of Thiobacillus ferrooxidans (a mesophile), an endospore forming moderate thermophile and an extremely thermophilic isolate. Following this, attempts were made to enhance copper tolerance of some isolated strains. A Thiobacillus sp. increased its resistance from 5 to 20 g/1 copper in the culture media. The results showed that all the important bacteria used for bioleaching, are present in Sarcheshmeh Copper Mine.
机译：描述了Sarcheshmeh铜矿的三大类铁和/或硫氧化原核生物（中温亲和性，中等嗜热性和极端嗜热性）的某些天然物种的分离，部分鉴定和铜耐受性。 NICICo（国家伊朗铜业公司）计划对该矿进行低品位铜矿的大量储藏（约2.8亿吨），因此考虑将其阐明该矿山的原生菌群，然后确定其耐受性。浸出条件，例如低pH值，高金属浓度和纸浆密度。从矿山的某些地方取样，并在确定的液体培养基中培养。分离了用于生物浸提的所有三个与温度有关的组的代表性菌株，从中获得了一些纯化的培养物。这些是铁氧化硫杆菌（嗜温菌），内生孢子形成中等嗜热菌和极嗜热菌的菌株。此后，人们尝试提高某些分离菌株的铜耐受性。硫杆菌属在培养基中将其抗性从5铜增加到20 g / 1铜。结果表明，Sarcheshmeh铜矿中存在用于生物浸出的所有重要细菌。

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