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首页> 外文会议>International conference on computational methods in science and engineering 2014 >Micronucleus Frequency and Hematologic Index in Colossoma macropomum (Pisces, Ariidae) for Environmental Impact Assessment at a Protected Area in Brazil
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Micronucleus Frequency and Hematologic Index in Colossoma macropomum (Pisces, Ariidae) for Environmental Impact Assessment at a Protected Area in Brazil

机译：巴西保护区环境影响评估中巨大巨像（双鱼座，ri科）的微核频率和血液学指标

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This study used micronucleus assays and erythrocyte indices in the freshwater fish tambaqui, Colossoma macropomum, to assess environmental impacts in the Environmental Protection Area at Maracana, Sao Luis, Brazil. Fish were sampled from two locations within the protected area, Serena Lagoon and Ambude River, on four occasions. Biometric data (length and weight) and an aliquot of blood were collected from each fish for analysis. Erythrocyte indices including: mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were calculated, and blood samples were examined for micronuclei and nuclear morphological changes. Micronuclei were found in fish from both locations, although the frequency was higher in fish from Ambude River. Nuclear morphological changes were identified only in fish collected from Ambude River. Several nuclear morphological changes were found in erythrocytes stained with Giemsa, including: micronuclei and binucleate nuclei. On average, erythrocyte indices were lower in fish collected from Ambude River than in those from Serena Lagoon. Our results indicate that micronuclei and erythrocyte indices can be used in C. macropomum as indicators of environmental health.
机译：这项研究使用淡水鱼坦巴基巨像Colossoma macropomum中的微核化验和红细胞指数来评估巴西圣路易斯马拉卡纳市环境保护区的环境影响。在保护区的两个地点塞雷纳泻湖和安贝德河上取样了四次。从每条鱼收集生物特征数据（长度和重量）和血液等分试样进行分析。计算红细胞指数，包括：平均红细胞体积，平均红细胞血红蛋白和平均红细胞血红蛋白浓度，并检查血液样本中的微核和核形态变化。在两个地点的鱼类中都发现了微核，尽管来自安布德河的鱼类的频率更高。仅在从安布德河（Ambude River）收集的鱼类中鉴定出核形态变化。在吉姆萨染色的红细胞中发现了几个核形态变化，包括：微核和双核核。平均而言，从安布雷德河采集的鱼的红细胞指数低于从塞雷纳泻湖的鱼。我们的结果表明，微核和红细胞指数可用于巨球藻作为环境健康的指标。

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