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首页> 外文期刊>Chemosphere >A screening test based on hematological and histological biomarkers to evaluate the environmental impacts in tambaqui (Colossoma macropomum) from a protected area in Maranhao, Brazilian Amazon
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A screening test based on hematological and histological biomarkers to evaluate the environmental impacts in tambaqui (Colossoma macropomum) from a protected area in Maranhao, Brazilian Amazon

机译：根据血液和组织学生物标记物进行的筛选测试，以评估巴西亚马逊州马拉尼奥保护区的坦巴基（大巨蟹）的环境影响

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Nowadays biomonitoring programs can benefit with mathematical models able to correlate biomarkers to monitor water pollution. The aim of this study was to develop a screening test based on hematological parameters and histological lesions in tambaqui (Colossoma macropomum), to allow the assessment of environmental impacts on fish inhabiting a protected area in Maranhao inside of Brazilian Amazon. Samples collected during three years (2012, 2013 and 2014) were grouped by season (dry and rainy) Water samples were also collected for physical chemistry analysis. Blood samples were stained with Acridine Orange to detect micronuclei and erythrocyte abnormalities. Gill tissues were stained with hematoxylin and counterstained with alcoholic eosin, and histopathological lesions were scored on a scale of 1-3, being 1 = minimal pathological importance, 2 = moderate pathological importance and 3 = marked pathological importance. A screening test for evaluating environmental impact was developed by fitting the measured data (necrosis, erythrocyte abnormalities, number of micronuclei) from tambaqui. A three-dimensional surface was fit to the empirical data. Our proposed model predicted the probability of necrosis (observed in euthanized animals) based on the numbers of micronuclei and abnormal erythrocytes (observed in blood samples from live animals) (correlation coefficient R = 0.89). The methodology could be applied for predicting contamination histories (chronic pollution that induces branchial lesions) in rivers using the micronucleus and erythrocyte abnormalities of the fishes (with a simple blood sample). (C) 2018 Elsevier Ltd. All rights reserved.
机译：如今，生物监测程序可以受益于能够关联生物标记物以监测水污染的数学模型。这项研究的目的是根据血液参数和坦巴基（Colossoma macropomum）的组织学损伤进行筛选试验，以评估环境对居住在巴西亚马逊河内马拉尼昂保护区的鱼类的环境影响。三年（2012年，2013年和2014年）收集的样品按季节（干燥和雨天）分组。还收集了水样品进行物理化学分析。血样用Orange啶橙染色以检测微核和红细胞异常。 he组织用苏木精染色，并用酒精曙红复染，组织病理学损伤按1-3评分，其中1 =最低病理学重要性，2 =中度病理学重要性和3 =明显病理学重要性。通过对来自坦巴基的测量数据（坏死，红细胞异常，微核数）进行拟合，开发了一种评估环境影响的筛选测试。三维表面适合于经验数据。我们提出的模型基于微核和异常红细胞（在活体动物的血液样本中观察到）的数量预测了坏死的概率（在安乐死的动物中观察到）（相关系数R = 0.89）。该方法可用于利用鱼类的微核和红细胞异常（用简单的血液样本）预测河流中的污染历史（引起分支损伤的长期污染）。 （C）2018 Elsevier Ltd.保留所有权利。

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