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首页> 外文会议>International Conference on Monitoring of Toxic Chemical and Biomarkers >Monitoring benzo(a)pyrene exposure using laser-excited Shpol'skii spectroscopy of benzo(a)pyrene metabolites
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Monitoring benzo(a)pyrene exposure using laser-excited Shpol'skii spectroscopy of benzo(a)pyrene metabolites

机译：使用激光激发的苯并（a）meta代谢物的Shpol'skii光谱法监测苯并（a）exposure的暴露

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Abstract: Exposure to polycyclic aromatic hydrocarbons (PAHs) is considered a serious threat to the health of animals and humans and should be thoroughly monitored. Next to chemical analysis of PAHs in the various environmental compartments, PAH metabolites in body fluids (e.g., bile and urine) could be measured to determine the actual uptake. Although pyrene is not considered particularly toxic, its metabolite 1-hydroxy pyrene is often used as a biomarker because it is usually found at considerable concentrations and the analysis is relatively simple. As the result of differences in volatility and/or solubility, the uptake of more relevant carcinogens like benzo(a)pyrene may be some orders of magnitude lower and is far more difficult to measure. Determination of benzo(a)pyrene metabolites requires a very selective and sensitive method, and so far these compounds could only be detected after exposure to heavy pollution. In this paper it will be shown how several hydroxy benzo(a)pyrene metabolites are selectively determined using Shpol'skii spectroscopy. With this method, highly resolved fluorescence spectra are obtained upon cooling the sample in a suitable solvent to cryogenic temperatures. When a tunable laser system is employed as an excitation source, sub-femtomole amounts can be detected. Applications of the technique to marine monitoring (benzo(a)pyrene metabolites in fish bile) and to occupational hygienics (benzo(a)pyrene metabolites in workers' urine) are discussed. The data will be compared with 1-hydroxy pyrene concentrations to evaluate the routine use of the latter compound as a biomarker.!28
机译：摘要：暴露于多环芳烃（PAHs）被认为是对动物和人类健康的严重威胁，应进行彻底监测。除了对各种环境区室中的PAHs进行化学分析外，还可以测量体液（例如，胆汁和尿液）中的PAH代谢物，以确定其实际摄入量。尽管pyr并不被认为具有特别的毒性，但其代谢物1-羟基often通常被用作生物标志物，因为通常以相当高的浓度发现and并且分析相对简单。由于挥发性和/或溶解度的差异，更相关的致癌物如苯并（a）py的吸收可能会降低几个数量级，并且更难测量。测定苯并（a）py代谢物需要非常选择性和灵敏的方法，到目前为止，这些化合物只有在受到严重污染后才能被检测到。在本文中，将显示如何使用Shpol'skii光谱法选择性地确定几种羟基苯并（a）re代谢物。使用这种方法，在合适的溶剂中将样品冷却至低温后，可获得高度分辨的荧光光谱。当采用可调激光系统作为激发源时，可以检测到亚飞摩尔量。讨论了该技术在海洋监测（鱼胆中苯并（a）py代谢物）和职业卫生（工人尿中苯并（a）py代谢物）中的应用。将数据与1-羟基pyr浓度进行比较，以评估后一种化合物作为生物标记物的常规用途。28

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