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首页> 外文会议>International Conference on Mushroom Biology and Mushroom Products; 20050408-12; Shanghai(CN) >Interspecific Hybridization of Ganoderma lucidum and G. tsugae by Protoplast Fusion
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Interspecific Hybridization of Ganoderma lucidum and G. tsugae by Protoplast Fusion

机译：原生质体融合对灵芝和虫的种间杂交

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Two strains of Ganoderma lucidum and one cultivar strain of G. tsugae were used as parents for interspecific hybridization. The 2-day old mycelia were protoplasted using 2 % Novozyme in 0.6 M mannitol to yield 2 x 10~5 protoplasts g~(-1) mycelium. Fusion of protoplasts was chemically induced by 30% polyethylene glycol. Then the protoplast mixture was plated onto regeneration medium with 0.6 M mannitol. Screening was carried out with the isolated protoplast regenerants by observing their growth rates at 18 and 37℃. The selected fast-growing hybrids showed unique DNA fingerprints by arbitrarily primed polymerase chain reaction. Also, they were somatically incompatible with the parents. Three hybrids were found fertile producing basidiomes and abundant basidiospores. From the sequences of nuclear interspacers 1 and 2 and mitochondrial small ribo-somal DNA, the 3 hybrids were genetically more similar with G. lucidum. Thus protoplast fusion can be applied in strain improvement of Lingzhi.
机译：两株灵芝菌株和一株杉菌株用作亲本间杂交。使用2％的Novozyme在0.6 M甘露醇中对2天大的菌丝体进行原生质体转化，产生2 x 10〜5个原生质体g〜（-1）菌丝体。 30％聚乙二醇化学诱导原生质体融合。然后将原生质体混合物铺在含0.6 M甘露糖醇的再生培养基上。通过观察分离的原生质体再生剂在18和37℃下的生长速率进行筛选。通过任意引发的聚合酶链反应，选定的快速生长的杂种表现出独特的DNA指纹。而且，他们在身体上与父母不相容。发现了三个杂种，它们产生可育的basidiomes和丰富的basidiospores。从核间隔子1和2以及线粒体小核糖体DNA的序列来看，这3个杂种在遗传上与灵芝相似。因此，原生质体融合可用于灵芝菌株的改良。

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