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首页> 外文会议>International Conference on Surface and Colloid Science; 200309; Brazil >Nanostructured layers of adsorbed collagen: conditions, mechanisms and applications
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Nanostructured layers of adsorbed collagen: conditions, mechanisms and applications

机译：吸附胶原蛋白的纳米结构层：条件，机理和应用

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The nanometer-scale organization of collagen (type Ⅰ) adsorbed on polystyrene (PS) and plasma-oxidized PS (PSox) was investigated using atomic force microscopy (AFM) as well as radio-assays and X-ray photoelectron spectroscopy. The surface properties induced by plasma treatment of PS were examined: PSox was more hydrophilic than PS, owing to oxygen incorporation, and was covered by a layer of polyelectrolyte which could be swollen by water. Collagen adsorbed on PS formed elongated structures, the density and the height of which increased with the adsorption duration. On PSox, a smooth and homogeneous collagen layer was formed after up to 2 h of adsorption, but elongated structures appeared after 24 h. A similar contrast was found between CH_3- and OH-termi-nated self-assembled monolayers, indicating the role of surface hydro-philicity. The adsorption duration affected the nanostructure of adsorbed collagen layers, not only through the adsorbed amount but also owing to reorganizations of the adsorbed phase. Compared with collagen adsorbed on PSox, collagen adsorbed on PS was more easily picked up for attachment to the AFM probe and more easily displaced along the surface plane by scanning with the atomic force microscope or by drying. This suggests that collagen molecules adsorbed on PS leave segments protruding into the solution, while they form a felt of lying molecules on PSox. The observed elongated structures may be due to association of collagen segments, and/or to particular interactions with the atomic force microscope probe. The respective importance of these two mechanisms may depend on the chemical nature of the substratum. Nanostructured collagen layers offer attractive perspectives to control mammalian cell response or as a template to create nanostructured polymer surfaces.
机译：利用原子力显微镜（AFM）以及放射分析和X射线光电子能谱研究了吸附在聚苯乙烯（PS）和等离子体氧化的PS（PSox）上的胶原蛋白（Ⅰ型）的纳米级结构。检查了通过等离子体处理PS所诱导的表面性能：由于氧的引入，PSox比PS亲水性更高，并且被一层可被水溶胀的聚电解质覆盖。吸附在PS上的胶原蛋白形成细长的结构，其密度和高度随吸附时间的延长而增加。在PSox上，最多吸附2小时后形成了光滑且均匀的胶原层，但24小时后出现了拉长的结构。在CH_3和OH终止的自组装单层膜之间发现了相似的对比，表明表面亲水性的作用。吸附持续时间不仅通过吸附量影响吸附胶原蛋白层的纳米结构，而且还由于吸附相的重组而影响。与吸附在PSox上的胶原蛋白相比，吸附在PS上的胶原蛋白更容易被拾取以附着到AFM探针上，并且更容易通过原子力显微镜扫描或干燥而沿表面平面移位。这表明吸附在PS上的胶原蛋白分子使片段突出到溶液中，而它们在PSox上形成了分子的毡状。观察到的细长结构可能是由于胶原蛋白片段的缔合和/或与原子力显微镜探针的特定相互作用所致。这两种机制的各自重要性可能取决于基质的化学性质。纳米结构的胶原蛋白层为控制哺乳动物细胞的反应提供了诱人的前景，也为创建纳米结构的聚合物表面提供了模板。

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