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首页> 外文会议>International Conference on Surface and Colloid Science; 200309; Brazil >Interaction between cationic bilayers and Candida albicans cells
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Interaction between cationic bilayers and Candida albicans cells

机译：阳离子双层分子与白色念珠菌细胞之间的相互作用

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摘要

The high-affinity adsorption of a cationic dioctadecyldime-thylammonium bromide (DODAB) bilayer to the yeast form of the fungal cell Candida albicans was studied as a function of DODAB dispersion method, DODAB concentration and cell number density (n) in pure water. As previously observed for bacteria, the number of liposomes adsorbed per cell decreased as a function of n, possibly owing to increased cell aggregation and reduction of the total cell area with n. The DODAB limiting adsorption was 1.9 x10~9 and 9.5 x 10~8 molecules'per cell for bilayer fragments and large vesicles, respectively, in water solution; the extraattractive hydrophobic interaction between hydrophobic regions of biomolecules and hydrophobic borders of bilayer fragments possibly accounting for this result. Furthermore, at 1 mM cationic lipid or surfactant, the percentage cell phosphate leakage induced by bilayer fragments, large vesicles or cetyltrimethylammonium bromide micelles was small, about 4-10%, and was consistent with previously published data on the absence of bacterial cell lysis. Electrophoretic mobilities for cells in the presence of both DODAB dispersions were consistent with desorption of large vesicles from cells; this desorption was absent for the bilayer fragments.
机译：研究了阳离子二十八烷基-溴化胸腺嘧啶（DODAB）双层对真菌细胞白色念珠菌的酵母形式的高亲和力吸附，它是DODAB分散方法，DODAB浓度和纯水中细胞数密度（n）的函数。如先前对细菌所观察到的，每个细胞吸附的脂质体的数量作为n的函数而减少，这可能是由于细胞聚集增加和n减少了总细胞面积。水溶液中双层碎片和大囊泡的DODAB极限吸附量为每个细胞1.9 x10〜9和9.5 x 10〜8个分子。生物分子的疏水区域和双层片段的疏水边界之间的吸引力疏水相互作用可能解释了这一结果。此外，在1 mM阳离子脂质或表面活性剂下，双层碎片，大囊泡或十六烷基三甲基溴化铵胶束引起的细胞磷酸盐泄漏的百分数很小，约为4-10％，与先前发表的关于不存在细菌细胞溶解的数据一致。在两种DODAB分散液存在下细胞的电泳迁移率与大囊泡从细胞中的解吸一致；双层碎片没有这种解吸。

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