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The effect of implemented pulsed electric field (PEF) treatment on the dehydrogenase activity of activated sludge

机译:实施脉冲电场(PEF)处理对活性污泥脱氢酶活性的影响

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The implementation of sludge disintegration in wastewater treatment processes is a possible strategy of sludge minimisation. Many sludge disintegration technologies are being explored. A new additional method of disintegration is the application of pulsed electric fields (PEF). In the PEF technique short high voltage pulses are applied to generate an electric field in the sample, which builds up an electrical potential difference between the inner and outer side of the membrane. By exceeding a critical potential a perforation of the membrane is evoked (Zimmermann et al. [1]). A benefit of PEF application on subsequent aerobic and anaerobic treatment processes is shown (Koners et al. [2], Kopplow et al[3]).rnIn this research the attention is turned to the release of cell content and change in bio-activity of the sludge after PEF implementation. The most common tests to determine the disintegrative effect is the release of dissolved organic carbon (DOC) or the uptake of chemical oxygen demand (COD). As to determine additionally sludge activity a dehydrogenase assay (DHA) with the oxidoreduction dye resazurin was chosen. The DHA data are not dependent on the metabolism of a nutritive additive like glucose. DHA can be a useful monitoring parameter for sludge activity, though it correlates not always to found respiration activity (Strotmann et al. [4]). According to Ewald et al. [5] DHA with resazurin generally is a quick test for cell viability.rnThe disintegrative effect of PEF implementation was tested by DOC and DHA and compared to each other. The test leads to the conclusion that for each energy input the fraction of inactivated micro-organism keeps in the same range, unaffected of the dry substance content. Consequently PEF implementation is more energy efficient when the content of dry substances is high.
机译:在废水处理过程中实施污泥分解是污泥最小化的可能策略。目前正在探索许多污泥分解技术。一种新的分解方法是脉冲电场(PEF)的应用。在PEF技术中,施加短的高压脉冲以在样品中产生电场,从而在膜的内侧和外侧之间建立电势差。通过超过临界电位,可引起膜穿孔(Zimmermann等人[1])。显示了PEF在随后的需氧和厌氧处理过程中的优势(Koners等人[2],Kopplow等人[3])。在这项研究中,注意力转向细胞含量的释放和生物活性的变化PEF实施后的污泥量。确定崩解效果的最常用方法是释放溶解的有机碳(DOC)或吸收化学需氧量(COD)。为了进一步确定污泥活性,选择了使用氧化还原染料刃天青的脱氢酶测定法(DHA)。 DHA数据不取决于营养添加剂(如葡萄糖)的代谢。尽管DHA并不总是与发现的呼吸活动相关,但DHA可以作为污泥活动的有用监测参数(Strotmann等人[4])。据埃瓦尔德等。 [5]含有刃天青的DHA通常是细胞活力的快速测试。通过DOC和DHA测试PEF实施的崩解效果,并将其相互比较。该测试得出的结论是,对于每种能量输入,灭活微生物的比例均保持在相同范围内,而不受干物质含量的影响。因此,当干燥物质的含量较高时,PEF的实施更加节能。

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