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首页> 外文会议>International Forum on Progress on Post-Genome Technologies(5'IFPT); 20070910-11; Suzhou(CN) >Determination of hepatoma-specific alpha-fetoprotein by a mini-column affinity chromatography method
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Determination of hepatoma-specific alpha-fetoprotein by a mini-column affinity chromatography method

机译：微型柱亲和色谱法测定肝癌特异性甲胎蛋白

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摘要

Cellular interaction and adhesion is a dynamic and integrated process requiring the participation of specialized cell surface receptors, structural proteins, signaling proteins, and the cellular cytoskeleton. In this paper, we choose a label-free and real-time method to measure and monitor cell adhesion on special microplates integrated with electronic cell sensor arrays. These plates were used in conjunction with the real-time cell electronic sensing (RT-CES?) system to dynamically and quantitatively monitor the specific interaction of cells. We do some treatment on them 24h after plant. It can be conclude that the system is sensitive enough to finger out some little changes of cells. In order to find out the mechanism of membrane fusion of cells, we use PEG(poly ethylene glycol) to make non-small lung cancer cells (A549 cells) fuse and plant them back into microwells on RT-CES, we can also get the changes from the plot of the experiment.
机译：细胞相互作用和粘附是一个动态的，整合的过程，需要专门的细胞表面受体，结构蛋白，信号蛋白和细胞骨架的参与。在本文中，我们选择了一种无标签的实时方法来测量和监视与电子细胞传感器阵列集成在一起的特殊微孔板上的细胞粘附。这些板与实时细胞电子传感（RT-CES？）系统结合使用，可以动态，定量地监测细胞的特定相互作用。植树后24小时，我们会对它们进行一些处理。可以得出结论，该系统足够灵敏，可以指出一些微小的细胞变化。为了找出细胞膜融合的机制，我们使用PEG（聚乙二醇）使非小肺癌细胞（A549细胞）融合，然后将它们植入RT-CES的微孔中，我们还可以得到实验图的变化。

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