DEVELOPMENT OF MICROSATELLITE MARKERS FROM TARTARY BUCKWHEAT

机译：苦荞微卫星标记的开发

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A genomic libraries enriched with (gT)_n repeats from Tartary buckwheat ( Fagopyrum tataricum) was constructed using 5' anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones form the libraries shown that all they contained microsatellites (totally 10 loci), and each was unique. Additional locus-specific primer was designed according the flanking sequence of loci. Two of the microsatellite loci of 10 Tartary buclwheat accessions were amplified using anchored primer and locus-specific primer, which gave a clear polymorphic pattern. The data confirmed that degenerate primer was reliably anchoring at the 5' end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of Tartary buckwheat.

机译：使用5'锚定PCR构建微卫星标记的富集了来自苦荞（Fagopyrum tataricum）的（gT）_n重复序列的基因组文库。从文库中对5个克隆进行测序分析，结果表明它们全部包含微卫星（总共10个位点），并且每个都是唯一的。根据基因座的侧翼序列设计了另外的基因座特异性引物。使用锚定引物和基因座特异性引物扩增了10个art藜鳞茎种质的两个微卫星基因座，这提供了清晰的多态性模式。数据证实简并引物可靠地锚定在微卫星的5'端，并且基于该技术开发的引物可用于苦荞的多样性分析。

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来源
《International Forum on Tartary Buckwheat Industrial Economy; 20060909-11; Taiyuan(CN)》|2006年|P.94-99|共6页
会议地点 Taiyuan(CN)
作者
Li Yanqin; Shi Tonglin; Zhang Zongwen;
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作者单位

Key Laboratory for Chemical Biology and Molecular Engineering of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China;

IPGRI Office for East Asia, c/o The Chinese Academy of Agricultural Sciences, Beijing 100081, China;

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会议组织
原文格式 PDF
正文语种 eng
中图分类荞麦;
关键词
microsatellite; fagopyrum tataricum; anchored PCR; degenerate primer;

机译：微卫星；荞麦；锚定PCR；简并引物;

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1. Development of microsatellite markers from tartary buckwheat [J] . Li YQ, Shi TL, Zhang ZW Biotechnology Letters . 2007,第5期

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2. Milling of microsatellite markers using next generation sequencing data and its application in genetic relationship analysis of tartary buckwheat (Fagopyrum tataricum Gaertn) [J] . KWANG-SOO CHO, YOUNG-HO YOON, SU-YOUNG HONG, Research on Crops . 2014,第3期

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3. Development of a DNA marker for variety discrimination specific to 'Manten-Kirari' based on an NGS-RNA sequence in Tartary buckwheat (Fagopyrum tataricum) [J] . Katsu Kenjiro, Suzuki Tatsuro, Fujino Kaien, Food Chemistry . 2019,第OCTa15期

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4. DEVELOPMENT OF MICROSATELLITE MARKERS FROM TARTARY BUCKWHEAT [C] . International Forum on Tartary Buckwheat Industrial Economy . 2006

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7. Development of microsatellite markers from tartary buckwheat [O] . Yan-qin Li, Æ Tong-lin Shi, Æ Zong-wen Zhang 2007

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DEVELOPMENT OF MICROSATELLITE MARKERS FROM TARTARY BUCKWHEAT

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