DEVELOPMENT OF MICROSATELLITE MARKERS FROM TARTARY BUCKWHEAT

机译：从鞑靼荞麦的微卫星标记的发展

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A genomic libraries enriched with (gT)_n repeats from Tartary buckwheat ( Fagopyrum tataricum) was constructed using 5' anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones form the libraries shown that all they contained microsatellites (totally 10 loci), and each was unique. Additional locus-specific primer was designed according the flanking sequence of loci. Two of the microsatellite loci of 10 Tartary buclwheat accessions were amplified using anchored primer and locus-specific primer, which gave a clear polymorphic pattern. The data confirmed that degenerate primer was reliably anchoring at the 5' end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of Tartary buckwheat.

机译：使用5'锚定PCR构建富含Tarrary荞麦（FagoPyrum Tataricum）的基因组文库（GT）_N的重复。 5克隆的测序分析形成图书馆的内容，它们包含微卫星（共10个基因座），每个都是独特的。根据基因座的侧面序列设计了另外的轨迹特异性底漆。使用锚固底漆和轨迹特异性引物扩增了10个Tarrary Bucle释放的微卫星基因座的两种微卫星基因座，其发出澄清的多态图案。数据证实，在微卫星的5'末端可靠地锚固的数据，并且基于该技术开发的引物可用于季后荞麦的分集分析。

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《International Forum on Tartary Buckwheat Industrial Economy》|2006年||共6页
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中图分类荞麦;
关键词
microsatellite; fagopyrum tataricum; anchored PCR; degenerate primer;

机译：微卫星;Fagopyrum tataricum;锚定PCR;退化底漆;

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1. Development of microsatellite markers from tartary buckwheat [J] . Li YQ, Shi TL, Zhang ZW Biotechnology Letters . 2007,第5期

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2. Milling of microsatellite markers using next generation sequencing data and its application in genetic relationship analysis of tartary buckwheat (Fagopyrum tataricum Gaertn) [J] . KWANG-SOO CHO, YOUNG-HO YOON, SU-YOUNG HONG, Research on Crops . 2014,第3期

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3. Development of a DNA marker for variety discrimination specific to 'Manten-Kirari' based on an NGS-RNA sequence in Tartary buckwheat (Fagopyrum tataricum) [J] . Katsu Kenjiro, Suzuki Tatsuro, Fujino Kaien, Food Chemistry . 2019,第OCTa15期

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4. DEVELOPMENT OF MICROSATELLITE MARKERS FROM TARTARY BUCKWHEAT [C] . Li Yanqin, Shi Tonglin, Zhang Zongwen International Forum on Tartary Buckwheat Industrial Economy; 20060909-11; Taiyuan(CN) . 2006

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7. Development of microsatellite markers from tartary buckwheat [O] . Yan-qin Li, Æ Tong-lin Shi, Æ Zong-wen Zhang 2007

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DEVELOPMENT OF MICROSATELLITE MARKERS FROM TARTARY BUCKWHEAT

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