• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Plant Nutrition Colloquium; 2005; Beijing >Expression of vacuolar Na~+/H~+ antiporters (ZmNHX) ami Na~+ exclusion in roots of maize (Zea mays L.) genotypes with improved salt resistance
【24h】

Expression of vacuolar Na~+/H~+ antiporters (ZmNHX) ami Na~+ exclusion in roots of maize (Zea mays L.) genotypes with improved salt resistance

机译：耐盐性提高的玉米基因型根中液泡型Na〜+ / H〜+反向转运蛋白（ZmNHX）和Na〜+的表达

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Among the most common impacts of soil salinity, Na~+ toxicity causes depression of vegetative growth in maize {Zea mays L.). Na~+ compartmentation into the vacuole mediated by vacuolar Na~+/H~+ antiporters can thus help preventing toxic Na~+ levels in the cytoplasm. In order to improve salt resistance, a salt-resistant maize inbred line (NaExIL) showing low Na~+ uptake at root level and low Na~+ translocation to the shoot was crossed with maize inbred lines that had been selected for resistance against osmotic stress. The obtained, new single-cross hybrids (SR hybrids) were then further screened in hydroponic culture (150 mM NaCl) and soil experiments (10 dS m~(-1)) over a period of 2 y. The most resistant SR hybrids, NaExIL and Pioneer 3906 were examined for their expression (real-time PCR) of vacuolar Na~+/H~+ antiporter genes in roots at cortex and stele level under controlled conditions in hydroponic culture (100 mM NaCl). The examined maize genotypes showed highest Na~+ concentrations in the cortex tissue of roots. The Na~+ concentrations in the stele were reduced genotype-specifically by 33% - 60%, and the shoots had the lowest Na~+ concentrations. There is evidence for a passive Na~+ exclusion step from cortex to stele at the level of the endodermis. Pioneer 3906, NaExIL and SR3 showed increased relative expression of ZmNHX in the cortex as well as in the stele. The remaining salt-resistant hybrids differed in their relative expression at the cortex and stele levels. Maize genotypes having the lowest stele Na~+ concentration (SR8, SR13) showed decreased, those with increased Na~+ concentration in the stele showed increased relative expression of ZmNHX isoforms in the same tissue.
机译：在土壤盐碱化的最常见影响中，Na〜+毒性导致玉米（Zea mays L.）营养生长降低。因此，由液泡状Na + + / H +反向转运蛋白介导的Na + +进入液泡中的分隔可以帮助防止细胞质中的Na + +毒性水平。为了提高耐盐性，将耐盐性玉米自交系（NaExIL）在根水平显示低的Na〜+吸收量，并将Na〜+易位到芽上，并与选择用于抵抗渗透胁迫的玉米自交系杂交。然后在2年的时间里，在水培法（150 mM NaCl）和土壤实验（10 dS m〜（-1））中进一步筛选获得的新的单杂交种（SR杂种）。在水培条件下（100 mM NaCl），在控制下，在皮质和碑ste水平的根中检测了抗性最强的SR杂交种NaExIL和Pioneer 3906在根中的液泡Na〜+ / H〜+反转运蛋白基因的表达（实时PCR）。 。玉米基因型在根皮层组织中表现出最高的Na〜+浓度。碑林中的Na〜+浓度在基因型上特异性降低了33％-60％，而枝条中的Na〜+浓度最低。有证据表明在皮层内从皮层到石碑的Na +排除步骤是被动的。先锋3906，NaExIL和SR3显示ZmNHX在皮层和碑骨中的相对表达增加。其余的耐盐杂种在皮层和石碑水平的相对表达有所不同。碑叶Na〜+浓度最低的玉米基因型（SR8，SR13）减少，而碑叶Na〜+浓度增加的玉米基因型在同一组织中的ZmNHX同工型的相对表达增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号