• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Symposium on Diagnosis and Control of Livestock Diseases Using Nuclear and Related Techniques 7-11 April 1997 in Vienna >Diagnosis of contagious caprine and contagious bovine pleuropneumonia by pcr and restriction enzyme analysis
【24h】

Diagnosis of contagious caprine and contagious bovine pleuropneumonia by pcr and restriction enzyme analysis

机译：通过pcr和限制性内切酶分析诊断传染性山羊和传染性牛胸膜肺炎

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Contagiou scaprine pleuropneumonia (CCPP) and contagious bovine pleuropneumonia (CBPP) are caused by Mycoplasma capricolum ssp. capripneumoniae and Mycoplasma mycoides ssp. mycoides, small colony type (SC), respectively. These species belong to the group of six mycoplasmas referred to as the Mycoplasma mycoides cluster. The members of the M. mycoides cluster are closely related, and some of them are vey difficult to grow. Diagnosis based on identification of the causative agents by cultivation, biochemical reactions, or serology is therefore difficult, and improved diagnostic methods are sorely needed. Identification methods for M. capricolum ssp. capripneumoniae and M. mycoides ssp. mycoides SC, based on detection of the 16S rRNA genes from all members of the M. mycoides cluster by polymerase chain reaction (PCR) and differentiation of species by restriction enzyme analysis with PstI and AluI, respectively, have been developed in the Department of Bacteriology, National Veterinary Institute Laboratory, Sweden.
机译：Contagiou scaprine胸膜肺炎（CCPP）和传染性牛胸膜肺炎（CBPP）是由分支支原体ssp引起的。 Capripneumoniae和Mycoplasma mycoides ssp。小菌落型（SC）的杀菌剂。这些物种属于被称为支原体支原体簇的六个支原体的组。蕈状支原体群的成员密切相关，其中一些很难生长。因此，基于通过培养，生化反应或血清学鉴定病原体的诊断是困难的，并且迫切需要改进的诊断方法。鉴定M. capricolum ssp。的方法capripneumoniae和M.mycoides ssp。细菌学系已经开发出了基于Mycoides SC的菌种，其基于通过聚合酶链反应（PCR）检测到Mycoides菌群所有成员的16S rRNA基因并通过分别用PstI和AluI进行限制性酶分析来区分物种。瑞典国家兽医研究所实验室。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号