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首页> 外文会议>International Symposium on Supercritical Fluids Tome 1: Natural Products, Process amp; Equipment Development; 20030428-20030430; Versailles; FR >SFE OF PHARMACOLOGICAL COMPOUNDS FROM Tabernaemontana catharinensis: ANALYSIS OF THE ANTIOXIDANT AND ANTIMYCOBACTERIAL ACTIVITIES
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SFE OF PHARMACOLOGICAL COMPOUNDS FROM Tabernaemontana catharinensis: ANALYSIS OF THE ANTIOXIDANT AND ANTIMYCOBACTERIAL ACTIVITIES

机译：中华绒螯蟹药理化合物的SFE：抗氧化剂和抗细菌活性的分析

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摘要

In the present work the pharmacological activities of SFE extracts from T. calharinensis were evaluated. The SFE extracts were obtained using supercritical CO_2 plus ethanol as cosolvent (4,6 and 9.2% m/m) at pressures of 250 and 300 bar and temperatures of 35, 45, and 55℃. The crude extracts were solvent-fractionated (hexanic fraction, alkaloid, and aqueous fractions) and analyzed by Thin-layer chromatography (TLC), and Gas-chromatography/Flame Ionization Detector (GC/ FID). The antioxidant and antimycobacterial activities were determined for the crude extracts and their fractions. The antioxidant activity was measured by the coupled reaction of limonene acid and p-carotene; the antimycobacterial activity against M. tuberculosis was measured by Microplate Alamar Blue Assay (MABA) technique. The results show that the minimum antioxidant activity was approximately 55% (after 3 h of reaction) while the maximum was 92% (at the first hour of reaction). The highest antimycobacterial activity was detected in the alkaloidal fraction (MIC - 128 μg/mL) while the aqueous fraction had the lowest antimycobacterial activity (MIC > 512 μg/mL),
机译：在目前的工作中，对T. calharinensis的SFE提取物的药理活性进行了评估。使用超临界CO_2加乙醇作为助溶剂（4,6和9.2％m / m）在250和300 bar的压力以及35、45和55℃的温度下获得SFE提取物。将粗提取物进行溶剂分离（己酸级分，生物碱和水相​​级分），并通过薄层色谱（TLC）和气相色谱/火焰电离检测器（GC / FID）进行分析。测定了粗提物及其馏分的抗氧化和抗分枝杆菌活性。通过柠檬烯酸和对-胡萝卜素的偶联反应来测量抗氧化活性。通过Microplate Alamar Blue Assay（MABA）技术测定了对结核分枝杆菌的抗分枝杆菌活性。结果表明，最小的抗氧化剂活性约为55％（反应3小时后），而最大值为92％（反应的第一小时）。在生物碱部分（MIC-128μg/ mL）中检测到最高的抗分枝杆菌活性，而在水相部分中具有最低的抗分枝杆菌活性（MIC> 512μg/ mL），

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