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首页> 外文会议>International Symposium on Supercritical Fluids Tome 1: Natural Products, Process amp; Equipment Development; 20030428-20030430; Versailles; FR >ANTIOXIDANT AND ANTIMYCOBACTERIAL ACTIVITIES OF BASIL (Ocimum gratissimum) EXTRACTS OBTAINED BY SFE
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ANTIOXIDANT AND ANTIMYCOBACTERIAL ACTIVITIES OF BASIL (Ocimum gratissimum) EXTRACTS OBTAINED BY SFE

机译：SFE获得的罗勒（Ocimum gratissimum）提取物的抗氧化剂和抗细菌活性

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摘要

The effects of fertilization and harvesting time on the global yield, antioxidant activity, and antimycobacterial activity for the system basil (Ocimum gratssimum) + CO_2 was studied. The SFE extracts were obtained at 40℃ and pressures of 100, 150, 200, 250, and 300 bar. The crude extracts were analyzed by Gas-chromatography/Mass-Spectrometry (GC-MS), and Gas-chromatography/Flame Ionization Detector (GC-FID). The antioxidant activity was assessed using the couple reaction of beta-carotene and linolenic acid, the antimycobacterial activity against M. tuberculosis was measured by the Microplate Alamar Blue Assay (MABA) technique. The global yields varied with the fertilization and harvesting period, and were pressure dependent: at 200 bar the highest yield (1.6%) was obtained from plants cultivated with no fertilization; for plants cultivated with fertilization the global yield increase continuously with pressure from 0.6 to 2% (100 to 300 bar). The major compounds in the extracts were eugenol (61%, area), beta-selinene (10%, area), trans-caryophyllene (3%, area), 1,8 cineol (1.5%, area), and alpha-selinene (3%, area). The antioxidant activities were above 90% for all extracts. The lowest MIC (64 μg/mL) was detected for the extract obtained at 250 bar.
机译：研究了施肥和收获时间对罗勒（Ocimum gratssimum）+ CO_2系统总产量，抗氧化活性和抗分枝杆菌活性的影响。 SFE萃取物是在40℃，100、150、200、250和300 bar的压力下获得的。粗提物通过气相色谱/质谱（GC-MS）和气相色谱/火焰电离检测器（GC-FID）进行分析。使用β-胡萝卜素和亚麻酸的偶联反应评估抗氧化活性，并通过Microplate Alamar Blue Assay（MABA）技术测量对结核分枝杆菌的抗分枝杆菌活性。全球产量随施肥和收获期的不同而变化，并且与压力有关：在200 bar的压力下，从未施肥的植物获得的最高产量（1.6％）。对于施肥栽培的植物，全球产量在0.6％至2％（100至300 bar）的压力下持续增加。提取物中的主要化合物是丁子香酚（61％，面积），β-selinene（10％，面积），反叶石竹烯（3％，面积），1,8 cineol（1.5％，面积）和α-selinene （3％，面积）。所有提取物的抗氧化活性均高于90％。在250 bar下获得的提取物的最低MIC（64μg/ mL）被检测到。

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