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首页> 外文会议>International Workshop on Trichoderma and Gliocladium; ; >Genetic diversity analysis of Magnaporthe grisea from some blast nurseries of Hunan province using random amplified polymorphic DNA
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Genetic diversity analysis of Magnaporthe grisea from some blast nurseries of Hunan province using random amplified polymorphic DNA

机译：湖南省一些高产苗圃稻瘟病菌遗传多样性的随机扩增多态性分析

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摘要

Diseased samples of Magnaporthe grisea were collected from Anhua, Taojiang, Songbai blast nurseries in Hunan province in 2001. In the previous study the each isolate was characterized by the mating type and Chinese pathotypes. Forty-four isolates tested were analyzed with 13 selected random amplified polymorphic DNA (RAPD) primers in the present work. The isolates tested were classified into 6 different genetic groups (lineages) by UPGMA using the software DPS 2000. Among them lineages Ⅳ and Ⅴ were the predominant one each with 11 isolates while lineage Ⅲ clustered only one isolate. The isolates with Mat 1.1 and Mat 1.2 belonging to different Chinese pathotypes were grouped in the same lineage; moreover isolates of each genetic lineage belonged to different Chinese physiological race. The results obtained confirm the genetic diversity and virulence complexity of rice blast fungus in Hunan province.
机译：2001年从湖南省安化市，桃江市，松柏州的苗圃中采集了稻瘟病菌的病原样品。在以前的研究中，每种分离株都具有交配型和中国致病型。在本工作中，使用13个选定的随机扩增多态性DNA（RAPD）引物分析了测试的44个分离株。 UPGMA使用DPS 2000软件将测试的分离株分为6个不同的基因组（谱系）。其中Ⅳ和Ⅴ谱系占优势，每一个分别有11个分离株，而Ⅲ谱系仅聚集了一个。属于不同中国病型的Mat 1.1和Mat 1.2的分离株归为同一谱系。此外，每个遗传谱系的分离物都属于不同的中国生理学种族。获得的结果证实了湖南省稻瘟病菌的遗传多样性和毒力复杂性。

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