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首页> 外文会议>International Young Researchers Conference: Physics, Technologies and Innovation >Genetic Diversity of E. Coli 0157:H7 Isolated from Aleppo River Water Samples Using Random Amplified Polymorphic DNA (RAPD) Marker
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Genetic Diversity of E. Coli 0157:H7 Isolated from Aleppo River Water Samples Using Random Amplified Polymorphic DNA (RAPD) Marker

机译：E. Coli 0157的遗传多样性：使用随机扩增多晶晶态DNA（RAPD）标记从Aleppo河水样品中分离的H7

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Waterborne outbreaks of diseases caused by pathogenic strains of E. coli have been described in many parts of the world. For the past five years in a row, water has been the main source of contamination. Aleppo River is highly polluted by the sewage water that transferred directly without processing. So, we conducted estimate total count of bacteria, the census total coliform group, also detected E. coli and especially serotype E. coli 0157:H7 in water samples that were taken from eight villages passed by the river. Our results showed significant differences between the collected samples, the total count of bacteria and total coliforms in the first site (Handarat) for water samples were the lowest, but in the last site (Jezraya) were the highest among all samples. Molecular characterization was done for eight strains of E. coli using the primer (COL-1) and eight strains of E. coli 0157:H7 using the primers (OPA-03, OPA-13 OPC-12, OPE-20) in RAPD. After gel documentation, we have got eight different bands from isolated strains of E. coli, and forty-five total bands with thirteen polymorphic bands from E. coli 0157:H7, also showed that primer OPA-13 was the best primer for detect E. coli 0157:H7. This article also showed that RAPD analysis had a high discriminatory capacity for typing E. coli isolates. Because of its simplicity and rapidity.
机译：在世界许多地区已经描述了由大肠杆菌的致病菌株引起的疾病的水性爆发。在过去五年中，水一直是污染的主要来源。 Aleppo River受到直接转移而无需加工的污水处理的高度污染。因此，我们进行了估计细菌的总计数，人口普查总大肠杆菌组，也检测到大肠杆菌，特别是血清型大肠杆菌0157：H7在河流通过的八个村庄中取出的水样中。我们的结果表明，收集的样品之间的差异显着差异，水样的第一个地点中的细菌总数和总大肠杆菌的差异是最低的，但在最后的网站（Jezraya）中是所有样品中最高的。使用底漆（OPA-03，OPA-13 OPC-12，OPE-20）在RAPD中使用底漆（COL-1）和8个大肠杆菌0157：H7的8个菌株的底Coli和8个菌株进行分子表征。 。在凝胶文件之后，我们已经获得了来自大肠杆菌的分离株的八种不同的条带，以及来自大肠杆菌0157：H7的四十五个多晶段的四十五个总带，也显示引物OPA-13是检测到e的最佳底漆。Coli 0157：H7。本文还表明，RAPD分析具有键入大肠杆菌分离株的高鉴别能力。因为它的简单性和快速。

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