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首页> 外文会议>Journal of Anhui Agricultural University >Cloning an Phylogenetic Analysis of Chitin Synthase Coding Genes of Penicillium chrysogenum
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Cloning an Phylogenetic Analysis of Chitin Synthase Coding Genes of Penicillium chrysogenum

机译：产黄青霉几丁质合成酶编码基因的系统进化分析

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摘要

DNA fragments homologous to chitin synthase were amplified from the genomic DNA fo penicillium chrysognum by PCR. Clonign and sequencing of the PCR-amplified fragments led to the identification of four different genes, designated PcCHSI, PcCHS2, PcCHS3, and PcCHS4. By comparison of the deduced amino acid sequences, PCCHSI was identified as a gene for class I chitin synthase, PcCHS2 and PcCHS3 were for class II, and PcCHS4 was for class III. For the phylogenetic study fo P.chrysogenum, the blast P similarity search and the bootstrap analysis fo the deduced amino acid sequences of the highly conserved regiion of chitin synthase genes were performed. The analysis of amino acid sequences implicateda close evoletionary relationship of penicillium to ascomycetours fungi, especially to genus aspergillus. The results from bootstrap analysis fo the chitin synthase also supported the usefulness of the chitin synthase for phylogenetic study of filamentous fungi.
机译：通过PCR从金黄色葡萄球菌的基因组DNA中扩增出与几丁质合酶同源的DNA片段。通过对PCR扩增片段进行克隆和测序，可以鉴定出四个不同的基因，分别称为PcCHSI，PcCHS2，PcCHS3和PcCHS4。通过比较推导的氨基酸序列，PCCHSI被鉴定为I类几丁质合酶的基因，PcCHS2和PcCHS3为II类，PcCHS4为III类。为了进行产黄青霉的系统发育研究，进行了blast P相似性搜索和bootstrap分析，以推导了几丁质合酶基因高度保守区域的氨基酸序列。氨基酸序列的分析暗示了青霉菌与子囊真菌，特别是曲霉属之间具有密切的驱除关系。几丁质合酶自举分析的结果也支持了几丁质合酶在丝状真菌系统发育研究中的有用性。

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