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首页> 外文会议>Journal of Huazhong Agricultural University （ Ziran Kexue Ban ) >Identification of Three Isometric Viruses from Pleurotus ostreatus (Running Title: ssRNA and dsRNA Viruses of Oyster Mushroom, Plerortus ostreatus)
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Identification of Three Isometric Viruses from Pleurotus ostreatus (Running Title: ssRNA and dsRNA Viruses of Oyster Mushroom, Plerortus ostreatus)

机译：鉴定三种来自平菇的等距病毒（运行名称：牡蛎蘑菇ssRNA和dsRNA病毒，Plerortus ostreatus）

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摘要

Natural viral epidemic was observed in the commercial farms of oyster mushroom, Pleurotus ostreatus , in Korea. The disease always accompanied the presence of isometric virus particles. Using purification procedures involving Tris-EDTA buffer extraction, PEG-NaCl precipitation, differential centrifugation, and equilibrium centrifugation in CsCl gradient (1.585 g/cm~3), we have obtained three isometric viral particles of 27 and two of 34 nm in diameter with different densities, suggesting that fruiting bodies were coinfected. The 27 nm particle, denser than 34 nm particle, was named oyster mushroom spherical virus (OMSV), encapsidated 2 different ssR-NAs of 6. 00 and 1.25 kb. There were two kinds of lighter particles with the same size of 34 nm; one, named oyster mushroom isometric virus-Ⅰ (OMIV-Ⅰ), which always coinfected with OMSV, encapsidated 12 different dsR-NAs of 2.65, 2.45, 2.40, 2.20, 2.15, 2.10, 2.05, 2.00, 1.90, 1.80, 1.00 and 0.80 kb. Another, named oyster mushroom isometric virus-Ⅱ (OM1V-Ⅱ), encapsidated 3 different dsRNAs of 2.25, 2.15, and 2.05 kb. Coom-assie Brilliant Blue stained polyacrylamide gel (12%) electrophoresis showed different polypeptides of coat proteins of Mr 29, 71, and 62 kDa in viral coat proteins of OMSV, OMIV-Ⅰ, and OMIV-Ⅱ particles, respectively. These results demonstrated that there are at least 3 different kinds of isometric viruses whose genomes were ssRNA or dsRNA in oyster mushroom.
机译：在韩国的牡蛎蘑菇平菇商业养殖场中观察到自然病毒流行。这种疾病总是伴随着等距病毒颗粒的出现。使用Tris-EDTA缓冲液提取，PEG-NaCl沉淀，差速离心和CsCl梯度（1.585 g / cm〜3）平衡离心的纯化程序，我们获得了三个等距的病毒颗粒，直径分别为27和两个，直径分别为34 nm不同的密度，表明子实体是共同感染的。密度大于34 nm的27 nm颗粒被称为牡蛎蘑菇球形病毒（OMSV），衣壳化了2种不同的ssR-NA，分别为6. 00和1.25 kb。有两种较轻的粒子，它们的大小相同，均为34 nm。一种名为牡蛎蘑菇等距病毒Ⅰ（OMIV-Ⅰ），总是与OMSV共同感染，衣壳化了12种不同的dsR-NA，分别为2.65、2.45、2.40、2.20、2.15、2.10、2.05、2.00、1.90、1.80、1.00和1。 0.80 kb。另一种名为牡蛎蘑菇等距病毒Ⅱ（OM1V-Ⅱ），衣壳化了3种不同的dsRNA，分别为2.25、2.15和2.05 kb。 Coom-assie艳蓝染色聚丙烯酰胺凝胶电泳（12％）显示OMSV，OMIV-Ⅰ和OMIV-Ⅱ颗粒的病毒外壳蛋白中Mr 29、71和62 kDa外壳蛋白的多肽分别不同。这些结果表明，牡蛎蘑菇中至少有3种不同的等距病毒，其基因组是ssRNA或dsRNA。

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