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首页> 外文会议>Journal of Huazhong Agricultural University （ Ziran Kexue Ban ) >Isolation and Identification of Gomphus Pur puraceus
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Isolation and Identification of Gomphus Pur puraceus

机译：紫菜的分离与鉴定

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摘要

Gomphus purpuraceus is an important rare mycorrhizal edible fungus. It is very difficult to identify the isolates by fruiting. The tissue isolation from different parts of different developmental phases of fruiting body and mycorrhiza was made with 5 media. The results showed that mycelia from fruiting body grew very slowly and thickly, mycelia from mycorrhiza and rhizomorph grew quickly and thinly. Out of 5 media, medium D was optimal medium for tissue isolation. The isolates from different resources were appraised through DNA fingerprinting in comparison with that of dry basidiocarps of G. purpuraceus, and RAPD patterns were sharp and stable by 8 random primers. The results suggested that isolates from fruiting body had the same DNA fingerprinting patterns as that of the basidiocarps tissues (such as pileus and stipe), whose similarity coeffecient was 0.884. However, the RAPD patterns of the isolates from mycorrhiza and rhizomorph were different from the others (including the basidiocarps tissues). These results further revealed that some isolates from fruiting body were real isolates of G. purpuraceus and the isolates from mycorrhiza and rhizomorph were not real isolates of the G. purpuraceus.
机译：紫癜是一种重要的罕见菌根食用菌。通过结果鉴定菌株是非常困难的。用5种培养基对子实体和菌根不同发育阶段的不同部位进行组织分离。结果表明，子实体的菌丝体生长缓慢而浓密，菌根和根茎的菌丝体生长较快而稀疏。在5种培养基中，培养基D是用于组织分离的最佳培养基。通过DNA指纹图谱与紫茎泽兰干枯果树相比，鉴定了来自不同资源的分离物，并且通过8个随机引物，RAPD图谱清晰且稳定。结果表明，来自子实体的分离株具有与基底果皮组织（如桩头和柄）相同的DNA指纹图谱，相似系数为0.884。但是，菌根和根状菌的分离株的RAPD模式与其他分离株（包括基底果皮组织）不同。这些结果进一步表明，来自子实体的一些分离物是紫癜的真正分离株，而菌根和根茎菌种的分离株不是紫癜的真正分离株。

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