CD25<'+> regulatory T cells (Tregs) mediate immune suppression through cell-cell contact with surface molecules, particularly cytotoxic T lymphocyte-associated antigen 4 (CTLA-4)'/> Expression of surface markers on peripheral CD4<'+>CD25<'high> T cells in patients with atopic asthma: role of inhaled corticosteroid
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首页> 外文会议>Journal of Organ Dysfunction: ISRD 2007 Abstract Book >Expression of surface markers on peripheral CD4<'+>CD25<'high> T cells in patients with atopic asthma: role of inhaled corticosteroid
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Expression of surface markers on peripheral CD4<'+>CD25<'high> T cells in patients with atopic asthma: role of inhaled corticosteroid

机译：特应性哮喘患者外周血CD4'+ CD25 <'high> T细胞表面标志物的表达：吸入糖皮质激素的作用

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摘要

Background CD4<'+>CD25<'+> regulatory T cells (Tregs) mediate immune suppression through cell-cell contact with surface molecules, particularly cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR), and transforming growth factor β (TGF-β), but little is known about the exact role of Tregs in the pathogenesis of asthma. This study sought to characterize the expression of surface markers on peripheral blood mononuclear cells derived Tregs in patients with atopic asthma and healthy subjects, and to investigate the role of inhaled corticosteroid on them. Methods Expression of surface molecules on CD4<'+>CD25 <'high> Tregs was detected by flow cytometry. The effect of inhaled corticosteroid on expression of the surface molecules on Tregs was determined in vivo and in vitro. Total serum immunoglobulin E (IgE) and high-sensitivity C-reactive protein were measured by enzyme linked immunosorbent assay and latex enhanced immunoturbidimetric assay, respectively. Results Equivalent numbers of peripheral Tregs were found in patients with atopic asthma (stable and acute) and healthy subjects. Tregs preferentially expressed CTLA-4, GITR, toll-like receptor 4 (TLR4), latency-associated peptide (LAP/TGF-β1), and forkhead box P3 (FOXP3). Patients with acute asthma had decreased numbers of CD4<'+>CD25<'high>LAP<'+> T cells relative to healthy subjects and stable asthmatics. Inhaled corticosteroid enhanced the percentage of Tregs expressing LAP in vivo and in vitro dose-dependently. Furthermore, the percentages of Tregs expressing LAP negatively correlated with total serum IgE levels and severity of asthma, while positively correlated with forced expiratory volume in one second percentage of the predicted value in patients with asthma. Conclusions Our data suggest that membrane-bound TGF-β1 is a potential candidate for predicting the severity of asthma, and may contribute to the sustained remission of asthma. Strategies targeting Treg cells on their surface markers, especially TGF-β1, are promising for future therapy of asthma.
机译：背景CD4 + + CD25 + +调节性T细胞（Tregs）通过细胞与表面分子，特别是细胞毒性T淋巴细胞相关抗原4（CTLA-4），糖皮质激素诱导的肿瘤坏死因子受体的接触来介导免疫抑制。家族相关蛋白（GITR）和转化生长因子β（TGF-β），但对Treg在哮喘发病中的确切作用了解甚少。这项研究旨在表征特应性哮喘患者和健康受试者外周血单核细胞Tregs上表面标志物的表达，并研究吸入糖皮质激素对其的作用。方法通过流式细胞术检测表面分子在CD4 + CD25-高Tregs上的表达。在体内和体外确定了吸入皮质类固醇对Tregs表面分子表达的影响。分别通过酶联免疫吸附法和乳胶增强免疫比浊法测定血清总免疫球蛋白E（IgE）和高敏C反应蛋白。结果在特应性哮喘（稳定和急性）患者和健康受试者中，外周血Treg的含量相等。 Tregs优先表达CTLA-4，GITR，toll​​样受体4（TLR4），潜伏期相关肽（LAP /TGF-β1）和叉头盒P3（FOXP3）。相对于健康受试者和稳定的哮喘患者，急性哮喘患者的CD4 + CD25-高LAP + T细胞数量减少。吸入皮质类固醇在体内和体外剂量依赖性地提高了表达LAP的Treg的百分比。此外，表达LAP的Treg的百分比与总血清IgE水平和哮喘严重程度呈负相关，而与强迫呼气量呈正相关，占哮喘患者预测值的1％。结论我们的数据表明膜结合的TGF-β1是预测哮喘严重程度的潜在候选者，可能有助于持续缓解哮喘。在其表面标志物，特别是TGF-β1上靶向Treg细胞的策略有望用于未来的哮喘治疗。

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  • 来源
  • 会议地点 Shanghai(CN);Shanghai(CN);Shanghai(CN);Shanghai(CN)
  • 作者

    Cao Shukun; rnXu Jing; rnShi Lei; rnZhang Heng;

  • 作者单位

    ZHANG Qian@Department of Respiratory Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China--YIN Kai-sheng@Department of Respiratory Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China--QIAN Fen-hong@Department of Respiratory Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China--LIU Hua@Department of Respiratory Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China--ZHOU Lin-fu@Department of Respiratory Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China--HUANG Mao@Department of Respiratory Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China--ZHANG Xi-long@Department of Respiratory Medicin;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng;chi
  • 中图分类 呼吸系及胸部疾病;
  • 关键词

    asthma; atopy; glucocorticoids; regulatory T cells; TGF-β;

    机译：哮喘;原子吸收;糖皮质激素;调节性T细胞;TGF-β;

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