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首页> 外文会议>Lab-on-a-Chip: Platforms, Devices, and Applications >Integrated chemical/biochemical sample collection, pre-concentration, and analysis on a digital microfluidic lab-on-a-chip platform
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Integrated chemical/biochemical sample collection, pre-concentration, and analysis on a digital microfluidic lab-on-a-chip platform

机译：在数字微流控芯片实验室平台上进行集成的化学/生化样品采集，预浓缩和分析

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摘要

An ideal on-site chemical/biochemical analysis system must be inexpensive, sensitive, fully automated and integrated, reliable, and compatible with a broad range of samples. The advent of digital microfluidic lab-on-a-chip (LoC) technology offers such a detection system due to the advantages in portability, reduction of the volumes of the sample and reagents, faster analysis times, increased automation, low power consumption, compatibility with mass manufacturing, and high throughput. We describe progress towards integrating sample collection onto a digital microfluidic LoC that is a component of a cascade impactor device. The sample collection is performed by impacting airborne particles directly onto the surface of the chip. After the collection phase, the surface of the chip is washed with a micro-droplet of solvent. The droplet will be digitally directed across the impaction surface, dissolving sample constituents. Because of the very small droplet volume used for extraction of the sample from a wide collection area, the resulting solution is relatively concentrated and the analytes can be detected after a very short sampling time (1 min) due to such pre-concentration. After the washing phase, the droplet is mixed with specific reagents that produce colored reaction products. The concentration of the analyte is quantitatively determined by measuring absorption at target wavelengths using a simple light emitting diode and photodiode setup. Specific applications include automatic measurements of major inorganic ions in aerosols, such as sulfate, nitrate and ammonium, with a time resolution of 1 min and a detection limit of 30 ng/m~3. We have already demonstrated the detection and quantification of nitroaromatic explosives without integrating the sample collection. Other applications being developed include airborne bioagent detection.
机译：理想的现场化学/生化分析系统必须便宜，灵敏，完全自动化和集成，可靠并与各种样品兼容。数字微流体芯片实验室（LoC）技术的出现提供了这样的检测系统，这是因为其具有便携性，减少样品和试剂的体积，更快的分析时间，增加的自动化，低功耗，兼容性的优点。大规模生产和高产量。我们描述了将样本收集集成到数字微流体LoC中的进展，该数字微流体LoC是级联撞击器设备的组成部分。通过将空气中的颗粒直接撞击到芯片表面来执行样品收集。在收集阶段之后，用微滴溶剂洗涤芯片表面。液滴将以数字方式引导穿过撞击表面，从而溶解样品成分。由于从宽广的采集区域中提取样品所用的液滴体积非常小，因此所得溶液相对浓缩，由于这种预浓缩，在很短的采样时间（1分钟）后即可检测到分析物。在洗涤阶段之后，将液滴与产生有色反应产物的特定试剂混合。通过使用简单的发光二极管和光电二极管设置测量目标波长处的吸收，可以定量确定分析物的浓度。具体应用包括自动测量气溶胶中的主要无机离子，例如硫酸盐，硝酸盐和铵盐，时间分辨率为1分钟，检测极限为30 ng / m〜3。我们已经证明了在不整合样品收集的情况下对硝基芳香族炸药的检测和定量。正在开发的其他应用包括机载生物制剂检测。

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