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Novel europium-tetracycline probe for phosphate determination in microtiter plate

机译:用于微量滴定板中磷酸盐测定的新型euro四环素探针

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A new luminescent europium probe is presented for the determination of phosphate (P) in microtiter plate format. The assay is based on the quenching of the luminescence of the europium-tetracycline 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays large Stokes' Shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7. The linear range of the calibration plot is from 5·10~(-6) mol/L to 7.5?10~(-4) mol/L of phosphate, and the limit of detection is 5 μmol/L.
机译:提出了一种新型发光euro探针,用于测定微量滴定板形式的磷酸盐(P)。该测定基于使用20.8μmol/ L的试剂浓度对phosphate-四环素1:1络合物的磷酸盐进行的猝灭。探针在400 nm处激发,并显示210 nm的大斯托克斯位移。发射最大值位于616 nm。该系统在中性pH 7时效果最佳。校准图的线性范围为5·10〜(-6)mol / L至7.5 to?10〜(-4)mol / L磷酸盐,并且检出限为5μmol/ L。

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