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首页> 外文会议>Laser-Tissue Interaction X: Photochemical, Photothermal, and Photomechanical >Methods of achieving three-dimensional reconstruction of tissue at the ultrastructural level demonstrating the distribution of melanosomes within retinal pigment epithelium
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Methods of achieving three-dimensional reconstruction of tissue at the ultrastructural level demonstrating the distribution of melanosomes within retinal pigment epithelium

机译：在超微结构水平实现组织三维重建的方法，表明视网膜色素上皮细胞中黑素体的分布

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摘要

Abstract: In predicting and measuring laser effect on retinal tissue for most of the visible to near infrared spectrum, one is concerned with the melanosome as the major absorber of incident energy. Differences in the location and density of melanosomes in the retinal pigment epithelium may have an impact on the effect of laser energy delivered to those tissues. Current models use estimates of numbers of melanosomes usually in an even distribution across a 5 - 8 micrometer deep volume. The goal of our study is to identify the three-dimensional distribution of melanosomes within the retinal pigment epithelium (RPE) for the use of those modeling laser tissue effects. We examined normal retinal pigment epithelium using three-dimensional (3-D) reconstruction from images obtained by transmission electron microscopy (TEM), light microscopy (LM) and confocal microscopy. Images were captured on a digital camera system attached to the microscope for both the transmission electron and light microscopy. Three-dimensional reconstruction was performed after digital deconvolution of microscopic images (Vaytek, Inc.). Three- dimensional images were then utilized for analysis of distribution of melanosomes and organelles within the pigment epithelial block. The distribution of melanosomes will be useful for accurate mathematical modeling of laser impact on the retina. !19
机译：摘要：在预测和测量大多数可见到近红外光谱对视网膜组织的激光作用时，人们将黑素体作为入射能量的主要吸收者。视网膜色素上皮细胞中黑素体的位置和密度的差异可能会影响传递到这些组织的激光能量的影响。当前模型使用的估计的黑素体数量通常分布在5至8微米深的体积中。我们的研究目标是确定视网膜色素上皮细胞（RPE）中黑素体的三维分布，以利用那些模拟的激光组织效应。我们从透射电子显微镜（TEM），光学显微镜（LM）和共聚焦显微镜获得的图像中使用三维（3-D）重建检查了正常视网膜色素上皮。在连接到显微镜的数码相机系统上捕获图像，用于透射电子和光学显微镜。在对显微图像进行数字反卷积后（Vaytek，Inc.）进行了三维重建。然后使用三维图像分析色素上皮块中黑素体和细胞器的分布。黑素体的分布将对激光对视网膜的影响进行精确的数学建模很有用。 ！19

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