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首页> 外文会议>Mechanisms for Low-Light Therapy; Progress in Biomedical Optics and Imaging; vol.7 no.26 >Wound healing stimulation in mice by low-level light
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Wound healing stimulation in mice by low-level light

机译：弱光刺激小鼠伤口愈合

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It has been known for many years that low levels of laser or non-coherent light (LLLT) accelerate some phases of wound healing. LLLT can stimulate fibroblast and keratinocyte proliferation and migration. It is thought to work via light absorption by mitochondrial chromophores leading to an increase in ATP, reactive oxygen species and consequent gene transcription. However, despite many reports about the positive effects of LLLT on wound healing, its use remains controversial. Our laboratory has developed a model of a full thickness excisional wound in mice that allows quantitative and reproducible light dose healing response curves to be generated. We have found a biphasic dose response curve with a maximum positive effect at 2 J/cm~2 of 635-nm light and successively lower beneficial effects from 3-25 J/cm~2, the effect is diminished at doses below 2J/cm~2 and gradually reaches control healing levels. At light doses above 25 J/cm~2 healing is actually worse than controls. The two most effective wavelengths of light were found to be 635 and 820-nm. We found no difference between filtered 635±15-nm light from a lamp and 633-nm light from a HeNe laser. The strain and age of the mouse affected the magnitude of the effect. Light treated wounds start to contract after illumination while control wounds initially expand for the first 24 hours. Our hypothesis is that a single brief light exposure soon after wounding affects fibroblast cells in the margins of the wound. Cells may be induced to proliferate, migrate and assume a myofibroblast phenotype. Our future work will be focused on understanding the mechanisms underlying effects of light on wound healing processes.
机译：多年来众所周知，低水平的激光或非相干光（LLLT）会加速伤口愈合的某些阶段。 LLLT可以刺激成纤维细胞和角质形成细胞的增殖和迁移。人们认为它是通过线粒体发色团的光吸收来起作用的，从而导致ATP，活性氧的增加以及随之而来的基因转录。然而，尽管有许多关于LLLT对伤口愈合的积极作用的报道，但其使用仍存在争议。我们的实验室建立了小鼠全切除伤口模型，该模型可产生定量和可再现的光剂量愈合反应曲线。我们发现了两相剂量响应曲线，在2 J / cm〜2的635-nm光下具有最大的正效应，而从3-25 J / cm〜2依次降低了有益效应，在低于2J / cm的剂量下效应减弱〜2并逐渐达到控制愈合水平。在高于25 J / cm〜2的光剂量下，愈合实际上比对照组差。发现两个最有效的光波长是635和820 nm。我们发现，灯发出的635±15 nm滤光和HeNe激光器的633 nm滤光没有区别。小鼠的应变和年龄影响了效应的大小。经过光照处理的伤口在光照后开始收缩，而对照伤口最初在最初的24小时内开始扩张。我们的假设是，受伤后不久的短暂短暂照射会影响伤口边缘的成纤维细胞。可以诱导细胞增殖，迁移并呈现成肌纤维细胞表型。我们未来的工作将集中在理解光对伤口愈合过程的潜在机制。

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