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首页> 外文会议>Mechanisms of photobiomodulation therapy XI >Photodynamic Antimicrobial Chemotherapy (PACT) Against Oral Microorganisms with the Use of Blue LED Associated to Curcumin
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Photodynamic Antimicrobial Chemotherapy (PACT) Against Oral Microorganisms with the Use of Blue LED Associated to Curcumin

机译：姜黄素相关的蓝色LED对口腔微生物的光动力抗菌化学疗法（PACT）。

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摘要

The use of curcumin as antimicrobial agent has been suggested and this effect may be potentialized by appropriate light. This study evaluated the effect of PACT using blue LED (λ450ηm ± 5nm, 220mW and spot of 0.785 cm~2) associated to Curcumin at different concentrations (75, 37.5, 18.75, 9.37 and 4.68 μg /mL). Microorganisms from the oral mucosa and the posterior region of the tongue were collected and inoculated into test tubes containing 8mL of TSB medium. For these assays were performed 16 readings. In the assays were used culture plate of 24 wells. To each well was added 400 uL of the suspension containing the microorganisms. Suspensions without curcumin were placed in eight wells. Elsewhere, curcumin was applied varying concentrations with pre-irradiation time of 5 min. After stirring, 200 μL aliquot was taken from each well and the readings were immediately carried out by a spectrophotometer (SPECTRA MAX). Assessments of turbidity were performed following CLSI standard methods. After 1 hour of incubation in a bacteriological oven, 200 μL aliquot was removed from the remaining wells for a second reading. The results showed a decrease of total microorganisms in the most of test groups. The best result of the PACT was with 75 μg/mL, showing 81% of inhibition. It is concluded that PACT with blue LED associated to Curcumin could be a potential mechanism for controlling microorganism proliferation on the oral cavity.
机译：已经提出使用姜黄素作为抗微生物剂，并且可以通过适当的光来增强这种作用。本研究使用与姜黄素不同浓度（75、37.5、18.75、9.37和4.68μg/ mL）关联的蓝色LED（λ450nm±5nm，220mW和0.785 cm〜2的斑点）评估了PACT的效果。收集来自口腔粘膜和舌后部的微生物，并将其接种到装有8mL TSB培养基的试管中。对于这些测定，进行16次读数。在测定中使用24孔的培养板。向每个孔中加入400uL的含有微生物的悬浮液。将没有姜黄素的悬浮液置于8个孔中。在其他地方，姜黄素的辐照时间为5分钟，浓度不同。搅拌后，从每个孔中取出200μL等分试样，并立即通过分光光度计（SPECTRA MAX）进行读数。浊度的评估是根据CLSI标准方法进行的。在细菌烤箱中孵育1小时后，从剩余的孔中取出200μL等分试样进行第二次读数。结果表明，大多数测试组中的总微生物量均减少。 PACT的最佳结果是75μg/ mL，显示出81％的抑制作用。结论是与姜黄素相关的带有蓝色LED的PACT可能是控制口腔微生物繁殖的潜在机制。

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  • 来源
    《Mechanisms of photobiomodulation therapy XI》|2016年|96950O.1-96950O.6|共6页
  • 会议地点 San Francisco CA(US)
  • 作者

    Fernando Jose Pires Sampaio; Gustavo M. Pires-Santos; Susana C. P. S. de Oliveira; Juliana S. C. Monteiro; Vanderlei S. Bagnato; Antonio L. B. Pinheiro;

  • 作者单位

    Center of Biophotonic, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil, 40110-150;

    Center of Biophotonic, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil, 40110-150;

    Center of Biophotonic, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil, 40110-150;

    Center of Biophotonic, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil, 40110-150,Department of Biology, Estate University of Feira de Santana, Feira de Santana, BA, Brazil, 44036-900;

    National Institute of Optics and Photonics, Sao Carlos, SP, Brazil, 13560-970;

    Center of Biophotonic, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil, 40110-150,National Institute of Optics and Photonics, Sao Carlos, SP, Brazil, 13560-970,Universidade Camilo Castelo Branco, Parque Tecnologico de Sao Jose dos Campos, Estr. Altino Bondesan, 500, Eugenio de Melo, Sao Jose dos Campos, SP, Brazil, 12247-016;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Lasers; Photodynamic Antimicrobial Chemotherapy; LED; Curcumin;

    机译：激光；光动力抗菌化学疗法；发光二极管;姜黄素;

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