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Diode laser and microvascular anastomosis-long term follow up

机译：二极管激光和微血管吻合术-长期随访

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Abstract: Direct diode laser assisted carotid end-to-end microanastomosis (LAMA) versus contralateral manual sutures microanastomosis (CMA) were performed in 70 Wistar rats. The laser beam-wavelength 830 nm, maximum power 3 W and continuous wave was transmitted through a micromanipulator and provided a focused spot of 300 $mu@m in diameter. After placement of three 10.0 stitches for edge coaptation, LAMA was achieved on left common carotid (0.8 - 1.2 mm) using laser shots (average 3) of 500 mW power, 4.5 s duration and 700 W/cm$+2$/ irradiance each. CMA was performed on right carotid by means of six 10.0 stitches. Light and scanning electron microscopy (n $EQ 82) showed in LAMA that re-endothelialization was complete on day 10 while collagenous fusion of media and adventitia was evident. The patency rate was 93% after LAMA versus 93% after CMA. The intra operative advantages of LAMA versus CMA: shorter operating time minimizing organ ischemia (13 min versus 22 min) and reduced endothelial trauma were evidenced. The technical advantages of diode laser were pointed out: small size of device, absence of water cooling system, no maintenance and decreasing price of diodes. !8
机译：摘要：在70只Wistar大鼠中，进行了直接二极管激光辅助的颈动脉端到端微吻合术（LAMA）与对侧手工缝合微吻合术（CMA）。激光束波长830nm，最大功率3W和连续波通过微操纵器传输并提供直径为300μm的聚焦点。放置3个10.0针进行边缘接合后，使用500 mW功率，4.5 s持续时间和700 W / cm $ + 2 $ /辐照度的激光照射（平均3次）在左颈总动脉（0.8-1.2 mm）上实现LAMA。 。通过六个10.0针对右颈动脉进行CMA。光和扫描电子显微镜（n EQ 82）在LAMA中显示，在第10天重新内皮化完成，而培养基和外膜的胶原蛋白融合明显。 LAMA后的通畅率为93％，而CMA后为93％。 LAMA与CMA相比具有术中优势：缩短手术时间可最大程度地减少器官缺血（13分钟比22分钟），并减少了内皮损伤。指出了二极管激光器的技术优势：装置尺寸小，没有水冷却系统，无需维护并且降低了二极管的价格。 ！8

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