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首页> 外文会议>Medical Imaging; Progress in Biomedical Optics and Imaging; vol.6 no.38 >Towards optical characterization of biological media: analysis of backscattered images in linearly polarized light, simulations and experiments
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Towards optical characterization of biological media: analysis of backscattered images in linearly polarized light, simulations and experiments

机译：致力于生物介质的光学表征：线性偏振光中的反向散射图像分析，模拟和实验

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Optical imaging is a non invasive way to characterise turbid media, which is of real interest for investigating biological tissues for diagnosis purposes. A method called Integral Reflectance (IR) has already been developed. The media being illuminated by a laser beam (670 nm, < 1 mW), the backscattered light is captured by a 2D CCD camera. The reduced scattering coefficient μ'_s and the absorption coefficient μ_a are determined from the image. Having μ'_s and μ_a, the objective is to improve the characterization by estimating the anisotropy factor g, using polarized light. Different patterns depending on g are produced in these images, presenting some lobes, centred in the entry point of the laser beam, whose number and shape vary with g. To assess a simple description of these patterns, a circular outline of the image, at a given radius, is studied by Fourier series decomposition, namely Fourier descriptors, whose indices, modulus and phase provide the number, the size and the orientation of the lobes, respectively. Backscattered images of turbid media with g in the range [0.006 ; 0.93] (μ'_s = 10, 20, 40 cm~(-1) ; μ_a = 0.01, 1, 5 cm~(-1)), were simulated using a Monte Carlo code for polarized light. Tables of Fourier descriptors were obtained as function of g, μ'_s and μ_a. Five reference solutions made of polystyrene spheres in liquid, with g varying from 0.71 to 0.919 (tissue phantoms) were tested. The Fourier descriptors were compared to simulations, and g could be retrieved with a maximum error of 10%.
机译：光学成像是表征混浊介质的一种非侵入性方法，这对于研究生物组织以进行诊断非常感兴趣。已经开发出一种称为积分反射率（IR）的方法。介质被激光束（670 nm，<1 mW）照射，后向散射光由2D CCD相机捕获。从图像确定减小的散射系数μ'_s和吸收系数μ_a。具有μ's和μ_a，目的是通过使用偏振光估计各向异性因子g来改善特性。在这些图像中产生取决于g的不同图案，呈现出一些裂片，其中心位于激光束的入射点，其数量和形状随g的变化而变化。为了评估这些模式的简单描述，通过傅立叶级数分解（即傅立叶描述符）研究给定半径处图像的圆形轮廓，其索引，模数和相位提供叶的数量，大小和方向， 分别。 g在[0.006;范围内]的混浊介质的反向散射图像。 0.93]（μ'_s= 10，20，40 cm〜（-1）；μ_a= 0.01，1，5 cm〜（-1）），是使用蒙特卡罗代码对偏振光进行模拟的。获得了作为g，μ's和μ_a的函数的傅立叶描述符表。测试了五种由聚苯乙烯球制成的液体参考溶液，其g在0.71至0.919（组织体模）之间变化。将傅立叶描述子与模拟进行比较，可以以10％的最大误差检索g。

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