Labeling GLUT4 with quantum dots for 3D tracking in live L6 cells

机译：用量子点标记GLUT4以在活L6细胞中进行3D跟踪

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Quantum dots (QDs) are light-emitting semiconductor nanoparticles with unique optical and spectroscopic properties, and are emerging as an alternative to conventional dyes for fluorescence-based applications. In this study, we have developed a procedure for labeling and imaging glucose transporter 4 (GUJT4) in living cells with streptavidin-conjugated quantum dot (QD-SA), and demonstrated that QD-SA contained in cytoplasm has no obvious negative influence on L6 cells. Insulin-stimulated GLUT4 translocation is central to glucose homeostasis. The results of this study have proved that QDs can be applied to observing the dynamics of GLUT4 translocation in vivo as a sensitive and long-term imaging probe.

机译：量子点（QD）是具有独特的光学和光谱特性的发光半导体纳米粒子，并且正在作为基于荧光的应用的常规染料的替代品而出现。在这项研究中，我们开发了标记和成像链霉亲和素共轭量子点（QD-SA）的活细胞中葡萄糖转运蛋白4（GUJT4）的程序，并证明细胞质中包含的QD-SA对L6没有明显的负面影响细胞。胰岛素刺激的GLUT4易位是葡萄糖稳态的关键。这项研究的结果证明，量子点可以作为一种敏感的长期成像探针，用于观察体内GLUT4易位的动力学。

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《World congress on medical physics and biomedical engineering;International congress of the IUPESM》|2011年|p.514-517|共4页
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作者
Feng Qu; Xiaoxuan Wang; Zubin Chen; Tao Liang; Anlian Qu;
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中图分类医用物理学;
关键词
QD-SA; GLUT4myc; internalization; imaging; translocation;

机译：QD-SA; GLUT4myc;内部化;成像易位;

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专利

1. Labeling and imaging of GLUT4 in live L6 cells with quantum dots [J] . Wang Xiaoxuan, Qu Feng, Chen Zubin, Biochemistry and Cell Biology . 2009,第4期

机译：带有量子点的活L6细胞中GLUT4的标记和成像
2. Labeling and imaging of GLUT4 in live L6 cells with quantum dots. [J] . Wang X, Qu F, Chen Z Biochemistry and Cell Biology . 2009,第4期

机译：带有量子点的活L6细胞中GLUT4的标记和成像。
3. Observing GLUT4 Translocation in Live L6 Cells Using Quantum Dots [J] . Anlian Qu, Feng Qu, Lingfeng Meng, Sensors . 2011,第2期

机译：使用量子点观察活L6细胞中的GLUT4易位
4. Labeling GLUT4 with quantum dots for 3D tracking in live L6 cells [C] . Feng Qu, Xiaoxuan Wang, Zubin Chen, World congress on medical physics and biomedical engineering . 2009

机译：标记Glut4与量子点，用于Live L6细胞中的3D跟踪
5. Dissecting the entry mechanism of targeting lentiviral vectors in living cells and developing quantum dot labeling of viruses for single virus tracking. [D] . Joo, Kye Il. 2009

机译：剖析了靶向慢病毒载体在活细胞中的进入机制，并开发了用于单个病毒跟踪的病毒量子点标记。
6. Observing GLUT4 Translocation in Live L6 Cells Using Quantum Dots [O] . Feng Qu, Zubin Chen, Xiaoxuan Wang, 2011

机译：使用量子点观察活L6细胞中的GLUT4易位
7. Observing GLUT4 Translocation in Live L6 Cells Using Quantum Dots [O] . Qu, Feng, Chen, Zubin, Wang, Xiaoxuan, 2011

机译：使用量子点观察活L6细胞中的GLUT4易位

Labeling GLUT4 with quantum dots for 3D tracking in live L6 cells

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