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首页> 外文会议>Asia-Pacific biochemical engineering conference;APBIOCHEC'97 >Construction and characterization of human dermal equivalent
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Construction and characterization of human dermal equivalent

机译：人皮肤等效物的构建和表征

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We developed a dermal equivalent (DE) which was engineered using human dermal dermal fibroblasts and a matrix of collagen gel. The in vitro construction of the DE was accomplished by casting a porcine collagen type I solution plus concentrated medium with isolated and cultured fibroblasts. These constructs were attached to culture dishes or left floating in culture medium. Contraction of attached gels resutls in decreased gel thickness without a change in gel diameter, and contraction of floating gels resutls in decreased gel thickness and diameter. After contraction, there was no increase in cell number in floating gels, but cells in attached gels began to increase after about 4 days of the lag phase in cell growth curve. At this lag phase, addition of fibroblast growth factor (FGF) at a concentration of 0.1 mug/ml promoted cell proliferation in the attached collagen gels, but no effect in floating gels. These results indicate that the method of contraction had an influence on the extracellular matrix (ECM) organization, and this influenced on not only cell growth but also fibroblast responsiveness to FGF. This suggests that attached collagen gel is more suitable as a dermal equivalent than the floating gel. And the final contracted area of attached gel is much larger than that of the floating gel since flating gel is contracted in all directions but attached gel is contracted only vertically.
机译：我们开发了一种真皮等效物（DE），该真皮等效物是使用人真皮真皮成纤维细胞和胶原蛋白凝胶基质设计的。 DE的体外构建是通过将猪I型胶原蛋白溶液和浓缩的培养基与分离的和培养的成纤维细胞一起浇铸而完成的。这些构建体附着在培养皿上或漂浮在培养基中。附着的凝胶的收缩使凝胶厚度减小而不改变凝胶直径，而漂浮的凝胶的收缩使凝胶厚度和直径减小。收缩后，漂浮凝胶中的细胞数量没有增加，但附着的凝胶中的细胞在细胞生长曲线的滞后阶段约4天后开始增加。在此滞后阶段，添加浓度为0.1杯/毫升的成纤维细胞生长因子（FGF）会促进附着的胶原蛋白凝胶中的细胞增殖，但对漂浮凝胶蛋白没有影响。这些结果表明，收缩方法对细胞外基质（ECM）的组织有影响，这不仅影响细胞生长，而且还影响成纤维细胞对FGF的响应性。这表明附着的胶原蛋白凝胶比浮动凝胶更适合用作真皮等效物。并且附着的凝胶的最终收缩面积比漂浮的凝胶的最终收缩面积大得多，这是因为平坦的凝胶在所有方向上都收缩，但是附着的凝胶仅在垂直方向上收缩。

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