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Evaluation of Recombinant Strains of Zymomonas mobilis for Ethanol Production from Glucose/Xylose Media

机译:发酵发酵单胞菌发酵菌株从葡萄糖/木糖培养基生产乙醇的评价

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The fermentation characteristics of two recombinant strains of Zymomonas mobilis, viz. CP4 (pZB5) and ZM4 (pZB5), capable of converting both glucose and xylose to ethanol, have been characterized in batch and continuous culture studies. The strain ZM4 (pZB5) was found to be capable of converting a mixture of 65 g/L glucose and 65 g/L xylose to 62 g/L ethanol in 48 h with a yield of 0.46 g/ g. Higher sugar concentrations resulted in incomplete xylose utilization (80 h) presumably owing to ethanol inhibition of xylose assimilation or metabolism. The fermentation results with ZM4 (pZB5) show a significant improvement over results published previously for recombinant yeasts and other bacteria capable of glucose and xylose utilization.
机译:运动发酵单胞菌的两个重组菌株的发酵特性。能够将葡萄糖和木糖都转化为乙醇的CP4(pZB5)和ZM4(pZB5)已在分批和连续培养研究中得到了表征。发现菌株ZM4(pZB5)能够在48小时内将65 g / L葡萄糖和65 g / L木糖的混合物转化为62 g / L乙醇,产量为0.46 g / g。较高的糖浓度可能导致不完全的木糖利用(80小时),这可能是由于乙醇抑制了木糖的同化或代谢。 ZM4(pZB5)的发酵结果显示,与以前公布的有关重组酵母和其他能够利用葡萄糖和木糖的细菌的结果相比,有了显着改善。

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