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首页> 外文会议>AIChE annual meeting >Impedance Array Studies of Mammalian Cell Growth
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Impedance Array Studies of Mammalian Cell Growth

机译：哺乳动物细胞生长的阻抗阵列研究

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Functional genomic studies and drug candidate testing both require high throughput, parallel experimentation to screen for variable cellular behaviors. Here we present the use of impedance sensing electrode array where each site is comparable to the area spanned by a few cells. The electrical signal from each sensing site provides information not only on cell “presence,” but also on the extent of cell attachment to the substrate. Data will be presented on the signals provided by mouse fibroblasts growing on 9 similar sized electrodes. In the absence of cells, each electrode’s impedance was found to depend as expected on electrode size and frequency. More notably, the impedance increased up to four-fold when fibroblasts attached and spread out over time. These findings suggest that such impedance sensors can detect cell “presence” as well as the extent of cell attachment to the substrate. Finite element simulation shows a good agreement with experimental results. From a technological perspective, impedance sensors could play a role in automating cell proliferation assays. Alternately, in structures with many sensors and sparse cell coverage, cell tracking and motility experiments may be potentially conducted. Finally, it may be possible to use such impedance measurements to screen mutants and malignant cells as well as effectors for altered adhesion prior to employing more demanding methods.
机译：功能基因组研究和候选药物测试都需要高通量，平行实验来筛选可变的细胞行为。在这里，我们介绍了阻抗感测电极阵列的使用，其中每个位置可与几个单元格所跨越的区域相媲美。来自每个感测部位的电信号不仅提供有关细胞“存在”的信息，而且还提供有关细胞附着在基质上的程度的信息。数据将显示在9个大小相似的电极上生长的小鼠成纤维细胞提供的信号上。在没有电池的情况下，发现每个电极的阻抗都取决于预期的电极尺寸和频率。更值得注意的是，当成纤维细胞附着并随时间散开时，阻抗增加到四倍。这些发现表明，这种阻抗传感器可以检测细胞的“存在”以及细胞附着在基底上的程度。有限元模拟表明与实验结果吻合良好。从技术角度来看，阻抗传感器可以在自动化细胞增殖测定中发挥作用。或者，在具有许多传感器且细胞稀疏覆盖的结构中，可能会进行细胞跟踪和运动性实验。最后，在采用更苛刻的方法之前，可以使用此类阻抗测量来筛选突变体和恶性细胞以及效应子，以检测粘附性是否发生改变。

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