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首页> 外文会议>TAPPI European PLACE conference >Formulation of an antimicrobial coating containing Nisaplin? intended for large scale production and inhibition of spoilage microorganisms
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Formulation of an antimicrobial coating containing Nisaplin? intended for large scale production and inhibition of spoilage microorganisms

机译：配制含有Nisaplin的抗微生物涂层？用于大规模生产和对腐败的微生物的抑制

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Antimicrobial food packaging could reduce food waste by extending shelf-life in addition to preserving food quality and safety. Utilization of the antimicrobial peptide Nisaplin?, which is an FDA GRAS approved additive, has the potential to be used in commercial antimicrobial food packaging applications. The objective of this study was to produce a Nisaplin containing coating formulated for large scale production equipment while maintaining efficacy. Differential scanning calorimetry (DSC) testing was conducted in order to determine a grade of polyvinyl alcohol (PVOH) and compatible plasticizer. Compatible plasticizers were determined based upon the plasticizers ability to lower the T_m (melt temperature) of the PVOH. Drawdowns were also produced from trial formulations to measure specific liquid coating characteristics and make observations of dried coated films. Percent solids (%) of liquid coatings and pH testing in additional to general observations were conducted. General observations of color, adhesion of the coating and seal testing were conducted on dry coated films. Film on lawn testing was conducted on dry coated films against Micrococcus luteus and Listeria monocytogenes. Control films did not contain Nisaplin. DSC testing revealed that glycerin lowered the melt temperature of partially hydrolyzed PVOH from 189.66°C (373.39°F) to 150.94°C (303.69°F) making the coating more suitable for sealing and less brittle. The pH was found to be between 4.0 and 4.5 for coatings containing Nisaplin (T) and 4.5-5.0 for control coatings (C) which did not contain Nisaplin. Average percent solids were 22.82% (T) and 20.63% (C). Coated films also achieved inhibition against M. luteus and L. monocytogenes compared to the control. The coated film was effective against a spoilage indicator organism in addition to a foodborne pathogen. Based on the characteristics of the coating and efficacy, it is possible to produce a commercial grade antimicrobial product containing Nisaplin that can extend the shelf-life of RTE food products.
机译：除了保存食品质量和安全外，抗菌食品包装还可以通过延长保质期来减少食物垃圾。利用抗微生物肽Nisaplinα，其是FDA GRAS批准的添加剂，具有用于商业抗微生物食品包装应用的可能性。本研究的目的是生产含有用于大规模生产设备的涂料的含NISAPLIN涂层，同时保持疗效。进行差扫描量热法（DSC）测试以确定聚乙烯醇（PVOH）和相容增塑剂等级。基于增塑剂降低PVOH的T_M（熔体温度）的增塑剂来确定相容的增塑剂。还通过试验制剂生产了缩减，以测量特定的液体涂层特性并进行干燥涂层薄膜的观察。进行液体涂料的固体（％）和在额外的一般观察中进行pH检测。一般的颜色观察，涂层的粘附和密封测试在干涂膜上进行。在针对微球菌和李斯特菌单核细胞生成的干涂膜上进行草坪测试的薄膜。对照薄膜不含Nisaplin。 DSC检测显示，甘油从189.66℃（373.39°F）至150.94℃（303.69°F）将部分水解PVOH的熔体温度降低至150.94℃（303.69°F），使涂层更适合密封和脆性。发现pH值在4.0和4.5之间用于含有Nisaplin（t）和4.5-5.0的涂层，用于对照涂层（c），其不含Nisaplin。平均固体百分比为22.82％（t）和20.63％（c）。与对照相比，涂覆的薄膜也抑制了对M.叶黄素和L.单核细胞生成的。除了食源性病原体之外，涂覆的膜还针对腐败的指示物生物有效。基于涂层和功效的特点，可以生产含有Nisaplin的商业级抗微生物产品，其可以延长RTE食品的保质期。

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