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首页> 外文会议>European Brewery Convention Congress >Development of molecular markers linked to powdery mildew resistance genes in hop (Humulus lupulus L.) to support breeding for resistance
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Development of molecular markers linked to powdery mildew resistance genes in hop (Humulus lupulus L.) to support breeding for resistance

机译：啤酒花（Humulus Lupulus L.）与粉状霉菌抗性基因有关的分子标记的发展，以支持抗性育种

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Powdery mildew is one of the most important fungal diseases of hop (Humulus lupulus). In this genome analytical research molecular markers could be identified to support the time consuming and difficult work of hop resistance breeding without modifying the genome. By the AFLP (amplified fragment length polymorphism) technique, a PCR based method, in addition with a special established leave infection test specific DNA-fingerprints could be detected associated with the presence of highly effective particular powdery mildew resistance genes from different wildhops. Using this markers the efficiency in selecting powdery mildew resistant seedlings can be increased. In addition a special differential display approach was performed to investigate the gene expression in hop seedlings to different time points after a powdery mildew infection. By this way resistant markers should be developed directly associated to genes responsible for the resistance reaction. First transcript derived fragments were found after different timepoints of infection.
机译：粉状霉菌是跳（Humulus Lupulus）最重要的真菌疾病之一。在这种基因组中，可以识别分析分子标记，以支持跳跃抗性育种的耗时和困难的工作而不改变基因组。通过AFLP（扩增的片段长度多态性）技术，可以检测到基于PCR基的方法，除了特殊建立的留下感染测试特异性DNA指纹，可以检测到来自不同野火的高效特定的粉状霉菌抗性基因。使用该标记，可以增加选择粉状霉菌耐药幼苗的效率。此外，进行特殊的差异显示方法以在白粉病感染后对啤酒花幼苗的基因表达研究。通过这种方式，应抗性标记与负责抗性反应的基因直接开发。在不同的感染时间点后发现了第一份转录物衍生片段。

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