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首页> 外文会议>Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production >Inheritance of two rapeseed mutants with apetalous flowers and molecular mapping of the gene(s) controlling petal-loss trait in Brassica napus
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Inheritance of two rapeseed mutants with apetalous flowers and molecular mapping of the gene(s) controlling petal-loss trait in Brassica napus

机译：两个带有油菜花的油菜突变体的遗传及控制甘蓝型油菜花瓣脱落性状的基因的分子定位

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In this study, APT02, a petal-loss mutant in Brassica napus and AM032, a petal-stamenoid mutant in Brassica campestris,were used to construct F2 and BC1 populations by crossed respectively with normal petalous rapeseed of Brassica napus and Brassica campestris. Mode of inheritance for two kinds of rapeseed mutants with apetalous flowers was studied. Based on the observation of PDgr (petalous degree) segregation, It was hypothesized that the petal-loss trait of APT02 was controlled by two pairs of recessive major genes and one pair of recessive modifying genes, and the petal-stamenoid trait of AM032 was controlled by a pair of recessive genes. Maternal effect wasn't found in apetalous trait of APT02 and AM032.An F2 population derived from a cross between apetalous line 'APT02'and normal petalled cultivar 'ZS NO.4' was used for molecular marker searching and chromosomal mapping of the gene(s) controlling petal-loss trait in Brassica napus.Twenty pairs of AFLP primers and 170 pairs of SRAP primers were selected and screened from two parents. In further selection through bulked segregant analysis(BSA)approach,one SRAP marker e8m3_4 (600 bp) and one AFLP marker E3247_15( 150 bp)were obtained and found to be linked to the gene(s) controlling petal-loss trait, with the genetic distance of 5 cM and 13.5 cM. A linkage map in Brassica napus was constructed. It consisted of 213 AFLP, 56 SSR loci and a morphology marker throughout 17 main linkage groups, two triplet and four linkage pairs. Total length of the map covered 2487.1 cM, and average interval between markers was 10.09 cM. By genetic mapping, the gene(s) controlling petal-loss trait (WHB) was mapped in LG4.
机译：本研究利用甘蓝型油菜和甘蓝型油菜的正常花瓣油菜杂交，利用甘蓝型油菜的花瓣丢失突变体APT02和甘蓝型油菜的花瓣-雄蕊突变体AM032构建了F2和BC1群体。研究了两种带花瓣的油菜突变体的遗传模式。根据对PDgr（花瓣度）分离的观察，假设APT02的花瓣脱落性状由两对隐性主基因和一对隐性修饰基因控制，而AM032的花瓣-类雄激素性状得到控制。由一对隐性基因组成。在APT02和AM032的无性状中没有发现母本效应.A2是由无性系'APT02'和正常花瓣品种'ZS NO.4'杂交而来的F2群体，用于分子标记搜索和该基因的染色体定位（ s）控制甘蓝型油菜的花瓣脱落性状。选择了20对AFLP引物和170对SRAP引物，并从两个亲本中进行了筛选。通过大量分离物分析法（BSA）进一步选择时，获得了一个SRAP标记e8m3_4（600 bp）和一个AFLP标记E3247_15（150 bp），并发现它们与控制花瓣脱落性状的基因相关，具有遗传距离为5 cM和13.5 cM。绘制了甘蓝型油菜的连锁图。它由213个AFLP，56个SSR基因座和一个形态标记组成，贯穿17个主要连锁组，两个三联体和四个连锁对。地图的总长度为2487.1 cM，标记之间的平均间隔为10.09 cM。通过遗传作图，将控制花瓣脱落性状（WHB）的基因定位在LG4中。

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