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首页> 外文会议>Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production >Fine mapping and cloning of the LepR3 blackleg (Leptosphaeria maculans) resistance gene in 'Surpass 400'
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Fine mapping and cloning of the LepR3 blackleg (Leptosphaeria maculans) resistance gene in 'Surpass 400'

机译：``Surpass 400''中LepR3黑腿病（Leptosphaeria maculans）抗性基因的精细定位和克隆

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'Surpass 400' was released as a blackleg resistant cultivar containing a single dominant blackleg resistance gene (LepR3) from Brassica rapa ssp. sylvestris. This study focused on the mapping and cloning of this resistance gene using a map-based cloning strategy. A consensus map was developed using SRAP (Sequence related amplified polymorphism) markers and a double haploid (DH) population developed from a cross of 'Westar' and 'Zhongyou 821'. 908 F2 and 2992 F3 individuals of 'Westar' ×'Surpass 400' were used to follow the segregation of disease resistance. One Mendelian gene controlled the disease resistance to blackleg as showed by trait segregation. Starting with the development of an anchoring marker on the ultra-density map, a fine map was constructed for this resistance gene that has over a hundred SRAP markers showing co-segregation with the resistance gene. After sequencing 7 SRAPs that are linked closely to the resistance gene, a region flanking the closest marker was targeted to develop SNPs from 'Westar' and 'Surpass 400' according to the Arabidopsis and B. rapa sequence. These SNPs were used to screen the recombinant population and helped to locate the candidate gene for LepR3. This gene is a leucine-rich repeat transmembrane protein kinase that may be involved in pathogen recognition. The complete sequences of this gene in 'Westar' and 'Surpass 400' showed that there is an insertion in 'Surpass 400' and a several different SNPs between 'Surpass 400' and 'Westar' alleles.
机译：“ Surpass 400”作为一种抗黑腿病品种发布，其中包含来自芸苔属的一种单一的显性黑腿病抗性基因（LepR3）。樟子松。这项研究集中于使用基于图谱的克隆策略对该抗性基因进行图谱和克隆。使用SRAP（序列相关的扩增多态性）标记物和从“ Westar”和“ Zhongyou 821”的杂交物中形成的双单倍体（DH）群体开发了一个共有图谱。 “ Westar”ד Surpass 400”的908个F2和2992个F3个体用于追踪疾病抵抗力的隔离。如性状分离所示，一个孟德尔基因控制了对黑腿病的抗病性。从超密度图上锚定标记的开发开始，为此抗性基因构建了一个精细图，该图具有一百多个SRAP标记，显示与该抗性基因共分离。对与抗性基因紧密连锁的7个SRAP进行测序后，根据拟南芥和B. rapa序列，将最接近标记侧翼的区域定位为从'Westar'和'Surpass 400'产生SNP。这些SNP用于筛选重组种群，并帮助定位LepR3的候选基因。该基因是富含亮氨酸的重复跨膜蛋白激酶，可能与病原体识别有关。该基因在“ Westar”和“ Surpass 400”中的完整序列表明，在“ Surpass 400”中有一个插入片段，在“ Surpass 400”和“ Westar”等位基因之间有几个不同的SNP。

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