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Development of Activity-based Cost Functions for Cellulase, Invertase, and Other Enzymes

机译:开发基于活动的纤维素酶,转化酶和其他酶的成本函数

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As enzyme chemistry plays an increasingly important role in the chemical industry, cost analysis of these enzymes becomes a necessity. In this paper, we examine the aspects that affect the cost of enzymes based upon enzyme activity. The basis for this study stems from a previously developed objective function that quantifies the tradeoffs in enzyme purification via the foam fractionation process (Cherry et al., Braz J Chem Eng 17:233-238, 2000). A generalized cost function is developed from our results that could be used to aid in both industrial and lab scale chemical processing. The generalized cost function shows several nonobvious results that could lead to significant savings. Additionally, the parameters involved in the operation and scaling up of enzyme processing could be optimized to minimize costs. We show that there are typically three regimes in the enzyme cost analysis function: the low activity prelinear region, the moderate activity linear region, and high activity power-law region. The overall form of the cost analysis function appears to robustly fit the power law form.
机译:随着酶化学在化学工业中的地位日益重要,对这些酶的成本进行分析成为必要。在本文中,我们研究了基于酶活性影响酶成本的各个方面。该研究的基础源于先前开发的目标函数,该目标函数量化了通过泡沫分离过程进行的酶纯化中的折衷(Cherry等,Braz J Chem Eng 17:233-238,2000)。根据我们的结果,可以开发出通用的成本函数,可用于工业和实验室规模的化学处理。广义成本函数显示了一些不明显的结果,可能会导致大量节省。另外,可以优化酶加工的操作和放大所涉及的参数以最小化成本。我们表明,在酶成本分析功能中通常有三种方案:低活性前线性区,中活性线性区和高活性幂律区。成本分析功能的整体形式似乎完全符合幂律形式。

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