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首页> 外文会议>International citrus congress >Effect of Arbuscular Mycorrhizal Fungi with Their Helper Microorganisms on Root Colonization and Growth of Trifoliate Orange Seedlings
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Effect of Arbuscular Mycorrhizal Fungi with Their Helper Microorganisms on Root Colonization and Growth of Trifoliate Orange Seedlings

机译：丛枝菌根真菌与辅助微生物对Trifoliate橙幼苗生长的影响

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摘要

Several kinds of helper microorganisms isolated from spore surfaces of Gigaspora margarita, an arbuscular mycorrhizal fungus (AMF) , have been reported to promote the growth of Gi. margarita by producing gaseous compounds including ethylene or non-gaseous compounds (Horii and Ishii, 2006). In this study, we investigated the effect of AMF with their helper microorganisms on AMF root colonization and growth of trifoliate orange seedlings. Ten days after the inoculation of Gi. margarita into one-yearold trifoliate orange seedlings, 4 kinds of helper microorganisms, Woollsia root associated fungus XV (Accession Number #AY230785) KHIMF1, Cladosporium cladosporioides KHIMF3, Bacillus asahii KHIMB1 and Methylobacterium sp. KHIMB2 were individually applied to the rhizospheres of the seedlings. Three months after the application, the seedlings were harvested. All of the helper microorganisms used increased the percentage of root colonization and total fresh weight of the seedlings. In particular, the inoculation of 3. 2×108 cells of B. asahii induced a remarkable stimulation on root colonization and tree growth compared with the control (Gi. margarita only). Our results show that the inoculation of AMF with their helper microorganisms is very useful to promote plant growth stimulation.
机译：据报道，从Gigaspora Margarita的孢子表面分离的几种辅助微生物，令人讨厌的菌根真菌（AMF）促进了GI的生长。通过生产乙烯或非气态化合物（Horii和Ishii，2006）的气态化合物来生产气体化合物。在这项研究中，我们研究了AMF对其辅助微生物的影响对AMF根定子和Trifoliate橙幼苗的生长。接种GI后十天。玛格丽塔进入一岁的Trifoliate橙色幼苗，4种辅助微生物，WOollsia根源XV（登录号＃AY230785）KHIMF1，Cladosporium cladosporioides Khimf3，Bacillus asahii Khimb1和甲基杆菌Sp。 KhimB2单独应用于幼苗的根茎。应用三个月后，幼苗被收获。所有的辅助微生物使用了幼苗的根部定植和总重量的百分比增加。特别地，与对照（GI.Margarita仅限）相比，Asahii的3. 2×108细胞的接种诱导了对根定子和树生长的显着刺激。我们的研究结果表明，随着辅助微生物的接种是促进植物生长刺激的非常有用。

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