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首页> 外文会议>International citrus congress >cDNA-AFLP Identifies Differently Expressed Transcripts of a Novel Late-ripening Citrus sinensis Mutant Potentially elated to Fruit Ripening
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cDNA-AFLP Identifies Differently Expressed Transcripts of a Novel Late-ripening Citrus sinensis Mutant Potentially elated to Fruit Ripening

机译：cDNA-AFLP识别不同表达的新型柑橘Sinensis突变体潜在地升高到果实成熟的转录物

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摘要

'Fengjiewancheng' (FJWC) is a late-ripening bud sport of 'Fengjie72-1' navel orange (Citrus sinensis Osbeck). In the present research, using cDNA-amplified fragment length polymorphism, we compared the transcript difference between fruits of the two varieties during ripening. As a result, about 60 000 clear transcript-derived fragments (TDFs) were generated with band sizes ranging from 50 to 500bp, of which 127 TDFs were down regulated (88.2%) and 17 were up-regulated (11.8%) in FJWC while compared with FJ72-1. Blast annotation indicated that 39 uniTDFs, which mainly located in mitochondria (26%)and membrane system (44%), were assigned with putative function in signal transduction, control of gene expression and carbohydrate biosynthesis, involving in extensive biological processes such as response to stimulus (12%), transport (12%), cellular metabolic process (12%), post-translational protein modification (12%), micromolecule biosynthesis (12%), transcription (10%), cellular localization (10%), phosphate metabolic process (10%)and developmental process (10% ). In addition, some TDFs showed relatively high identities with known functional genes and had differential expression patterns in fruit pulp or peel between FJWC and FJ72-1 during fruit ripening, implying a potential role in the formation of the late-ripening phenotype.
机译：'凤庚海城'（FJWC）是'凤杰72-1'肚脐橙（柑橘Sinensis Osbeck）的后期成熟芽运动。在本研究中，使用cDNA扩增的片段长度多态性，我们比较了在成熟过程中两种品种的果实之间的转录物差异。结果，用50至500bp的带尺寸产生约60 000个透明的转录物衍生片段（TDF），其中127个TDF在下调（88.2％）和17个在FJWC中调节（11.8％）与FJ72-1相比。 BLAST注释表明，主要位于线粒体（26％）和膜系统（44％）的39个UNERDF，用于指定功能在信号转导，控制基因表达和碳水化合物生物合成中，涉及广泛的生物过程，如响应刺激（12％），运输（12％），细胞代谢过程（12％），翻译后蛋白质改性（12％），微麻骨合成（12％），转录（10％），细胞定位（10％），磷酸盐代谢过程（10％）和发育过程（10％）。此外，一些TDFS显示出具有已知的功能基因的相对高的身份，并且在果实成熟期间FJWC和FJ72-1之间的果肉或果皮中具有差异表达模式，这意味着在后期成熟表型中的形成中的潜在作用。

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