首页> 外文会议>Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009 >Application of the 50-mer Oligonucleotide Arrays Hybridization for Profiling Microbial Community in Acid Mine Drainage Sediment
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Application of the 50-mer Oligonucleotide Arrays Hybridization for Profiling Microbial Community in Acid Mine Drainage Sediment

机译:50聚体寡核苷酸阵列杂交技术在酸性矿山排水沉积物微生物群落分析中的应用

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Microbial community compositions and functional activities of three acid mine drainage (AMD) sediment samples from different sites in Dexing copper mine were analyzed by 50- mer oligonucleotide arrays which have been constructed and evaluated. The results showed that microbial community was composed of 15 different genus or species, including Acidithiobacillus ferrooxidans(A. ferrooxidans), Leptospirillum sp., Acidiphilum spp., Acidithiobacillus thiooxidans, Acidobacteria, Acidithiobacillus caldus, Sulfobacillus sp., Desulfovibrio longus, Syntrophus sp., metallosphaera, Alicyclobacillus spp., Holophaga sp., Sulfolobus sp., Thiomonas spp. and Actinomyces naeslundii. Acidiphilum was abundant in three samples, for the anaerobic environment of sediment could inhibited aerobic bacteria, such as A. ferrooxidans. Heterotrophic Acidiphilium is able to catalyze the dissimilatory reduction of ferric iron to ferrous even in the presence of small amounts of oxygen. Among all the samples, the diversity was high in samples (site YTW and ZJ) containing low pH while the diversity was low in sample (site KZX) containing very high pH. Microarray hybridization data indicated that iron metabolism functional gene, Sulfur metabolism functional gene and metal-resistance gene and other genes existed in the AMD system. At all samples examined, microbial community and function activities were positively correlated with pH.
机译:通过构建和评估的50聚体寡核苷酸阵列分析了德兴铜矿不同地点的三个酸性矿山排水(AMD)沉积物样品的微生物群落组成和功能活性。结果表明,微生物群落由15种不同的属或物种组成,包括嗜酸性嗜酸硫杆菌(A. ferrooxidans),钩端螺旋体属菌,嗜酸杆菌属,嗜酸硫杆菌属,嗜酸细菌,嗜酸嗜酸芽孢杆菌,嗜硫酸杆菌属,长嗜脱硫杆菌, ,金属球菌,脂环芽孢杆菌属,霍洛帕加菌属,硫磺菌属菌,硫门氏菌属。和内生放线菌。在三个样品中,嗜酸杆菌含量很高,因为沉积物的厌氧环境可以抑制好氧细菌,如氧化铁农杆菌。即使在存在少量氧气的情况下,异养性嗜酸碱菌也能催化将三价铁还原为亚铁。在所有样品中,pH值较低的样品(YTW和ZJ部位)的多样性较高,而pH值很高的样品(KZX部位)的多样性较低。芯片杂交数据表明,AMD系统中存在铁代谢功能基因,硫代谢功能基因和金属抗性基因等基因。在所有检查的样品中,微生物群落和功能活动与pH值呈正相关。

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