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首页> 外文会议>Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009 >Resveratrol Induces Human Hepatoma Cancer Cells HepG-2 Apoptosis via Mitochondria Pathways
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Resveratrol Induces Human Hepatoma Cancer Cells HepG-2 Apoptosis via Mitochondria Pathways

机译：白藜芦醇通过线粒体途径诱导人肝癌细胞HepG-2凋亡。

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To study mechanism of resveratrol induce tumor cell apoptosis through mitochondria pathways. Measurements using mononuclear cell direct cytotoxicity assay shows that its cytotoxic effect on HepG-2 is strong with IC50 being 34.86 mumolldrL-1. Measurement of action of apoptosis using morphology with Hoechst 33258 stain show that karyoplasmic ratio of tumor cells diminish, cell nucleus pyconsis, the staining extent become deeper, chromatin prosperous aggregation, fragmentation. The cells breaks up into several apoptotic bodies of different sizes. As RES concentration is increased, these morphological changes under microscope become more and more clear, indicating that the proportion of cells undergoing apoptosis is gradually increasing. Measurement tumor cell cycle dealed with RES using flow cytometry shows that RES leads to a decrease in the proportion of cells in G0/G1 phase, an increase in the proportion of cells in the S phase and decrease in the proportion of cells in G2/M phase.This shows that RES has influence in thetumor cell cycle and can block tumor cells from S phase to G2/M phase.At sametime, The rate of apoptosis is26.130%,31.599%,65.655%.Measurement mitochondrial membrane electric potential(Deltapsim) treated with RES using the flow cytometry with Rodanmin 123 stain shows that RES can obviously cut down the Deltapsim of the tumor cells with the increasement of the dose. This effect shows dose-dependent. This effect shows dose-dependent too.In a word, RES can induce tumor cell apoptosis via mitochondria pathways.
机译：研究白藜芦醇通过线粒体途径诱导肿瘤细胞凋亡的机制。单核细胞直接细胞毒性实验表明，其对HepG-2的细胞毒性作用强，IC 50 为34.86 mumolldrL -1 。用Hoechst 33258染色的形态学测量细胞凋亡的作用表明，肿瘤细胞的核质比例减少，细胞核呈脓性，染色程度变深，染色质繁荣聚集，破碎。细胞分裂成几个大小不同的凋亡小体。随着RES浓度的增加，在显微镜下这些形态变化变得越来越清晰，表明经历凋亡的细胞比例逐渐增加。流式细胞术检测RES对肿瘤细胞周期的影响，结果表明RES导致G 0 / G 1 期细胞比例降低，而RES比例升高。 RES对肿瘤细胞周期有影响，可以阻断肿瘤细胞从S期到G 2 / M期。同时，凋亡率分别为26.130％，31.599％，65.655％。采用Rodanmin 123染色的流式细胞仪检测RES处理后的线粒体膜电位（Deltapsim），结果表明RES可以明显降低肿瘤细胞的Deltapsim。随着剂量的增加。这种作用显示出剂量依赖性。这种作用也显示出剂量依赖性。总之，RES可以通过线粒体途径诱导肿瘤细胞凋亡。

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