Cloning, Characterization and Application of the Promoter Region of the Alkaline Protease Gene in Bacillus alcalophillus PB92

机译：碱性芽孢杆菌PB92中碱性蛋白酶基因启动子区的克隆，鉴定及应用

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Promoter fragment of alkaline protease gene was cloned from Bacillus alcalophillus PB92 genome by TAIL-PCR. The fragment was sequenced and analyzed, then submitted to GenBank (EU130686). The results showed that it contained several typical promoter charactered regions and two reverse translation frames located in - 538 to - 370 bp and - 275 to - 128 bp region. Deletion analysis of the sequence indicated that 414 bp upstream of the TSS exhibited predominant promoter activity, while an 105 bp length could serve as this function. Additionally, our data demonstrated that a representative Sec- type signal peptide structure presented in PB92 alkaline protease signal peptide. The efficiency of P_aprE- AprE signal peptide gene cassette was validated by its driving a plant sweet protein monellin gene highly expression in Bacillus subtilis 1A751.

机译：通过TAIL-PCR从碱性芽孢杆菌PB92基因组中克隆了碱性蛋白酶基因的启动子片段。对该片段进行测序和分析，然后提交给GenBank（EU130686）。结果表明，它包含几个典型的启动子特征区域和位于-538至-370 bp和-275至-128 bp区域的两个反向翻译框。对该序列的缺失分析表明，TSS上游414bp显示出主要的启动子活性，而105bp的长度可以起到该功能。此外，我们的数据表明，PB92碱性蛋白酶信号肽中存在代表性的Sec型信号肽结构。 P -AprE信号肽基因盒的效率通过其驱动植物甜蛋白莫内林基因在枯草芽孢杆菌1A751中高表达而得到验证。

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《Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009》|2009年|1-4|共4页
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Kun Chen; Yong Jiang; Nan Wang; Xuegang Luo; Fuping Lu; Tongcun Zhang;
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genomics; molecular biophysics; proteins; Bacillus alcalophillus PB92; GenBank; TAIL-PCR; alkaline protease gene; cloning; genome; plant sweet protein monellin gene; promoter region;

机译：基因组学;分子生物物理学;蛋白质;碱性芽孢杆菌PB92; GenBank; TAIL-PCR;碱性蛋白酶基因;克隆;基因组;植物甜蛋白莫奈林基因;启动子区;

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1. Cloning, expression and characterization of a novel alkaline serine protease gene from native Iranian Bacillus sp.; a producer of protease for use in livestock [J] . Ariyaei Ahmadreza, Farhadi Ayoub, Moradian Fatemeh, Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function . 2019,第期

机译：来自天然伊朗芽孢杆菌新碱性丝氨酸蛋白酶基因的克隆，表达和表征。用于牲畜的蛋白酶生产商
2. Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene. [J] . J C van der Laan, G Gerritse, L J Mulleners, Applied and Environmental Microbiology . 1991,第4期

机译：芽孢杆菌碱性蛋白酶基因的克隆，鉴定和多重染色体整合。
3. Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. [J] . N Vasantha, L D Thompson, C Rhodes, Journal of bacteriology . 1984,第3期

机译：来自解淀粉芽孢杆菌的碱性蛋白酶和中性蛋白酶的基因在编码信号序列和成熟蛋白的区域之间包含一个大的开放阅读框。
4. Cloning,characterization and application of the promoter region of the alkaline protease gene in Bacillus alcalophillus PB92 [C] . Kun Chen, Yong Jiang, Nan Wang, The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议）论文集 . 2009

机译：碱性芽孢杆菌PB92碱性蛋白酶基因启动子区的克隆，表征及应用
5. Molecular cloning and characterization of the 5'-flanking and promoter region of the human dopamine D5 receptor gene. [D] . Beischlag, Timothy Victor Peter Cresswell. 1996

机译：人多巴胺D5受体基因5'侧翼和启动子区域的分子克隆和表征。
6. Cloning characterization and multiple chromosomal integration of a Bacillus alkaline protease gene. [O] . J C van der Laan, G Gerritse, L J Mulleners, 1991

机译：芽孢杆菌碱性蛋白酶基因的克隆鉴定和多重染色体整合。
7. Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene [O] . J C van der Laan, G Gerritse, L J Mulleners, 1991

机译：芽孢杆菌碱性蛋白酶基因的克隆，表征和多染色体整合
8. Cloning, Nucleotide Sequence, and Regulation of the Bacillus subtilis gpr Gene,Which Codes for the Protease That Initiates Degradation of Small, Acid-Soluble Proteins during Spore Germination [R] . Sussman, M. D., Setlow, P. 1991

机译：枯草芽孢杆菌gpr基因的克隆，核苷酸序列和调控，其编码在孢子萌发期间开始降解小的酸溶性蛋白质的蛋白酶

Cloning, Characterization and Application of the Promoter Region of the Alkaline Protease Gene in Bacillus alcalophillus PB92

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