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Real-time mapping of the corneal sub-basal nerve plexus by in vivo laser scanning confocal microscopy

机译:通过体内激光扫描共聚焦显微镜实时绘制角膜下基底神经丛

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The aim of the study was to produce two-dimensional reconstruction maps of the living corneal sub-basal nerve plexus by in vivo laser scanning confocal microscopy in real time. CLSM source data (frame rate 30Hz, 384x384 pixel) were used to create large-scale maps of the scanned area by selecting the Automatic Real Time (ART) composite mode. The mapping algorithm is based on an affine transformation. Microscopy of the sub-basal nerve plexus was performed on normal and LASIK eyes as well as on rabbit eyes. Real-time mapping of the sub-basal nerve plexus was performed in large-scale up to a size of 3.2mm x 3.2mm. The developed method enables a real-time in vivo mapping of the sub-basal nerve plexus which is stringently necessary for statistically firmed conclusions about morphometric plexus alterations.
机译:该研究的目的是通过体内激光扫描共聚焦显微镜实时制作活的角膜基底下神经丛的二维重建图。通过选择自动实时(ART)合成模式,使用CLSM源数据(帧频30Hz,384x384像素)创建扫描区域的大规模地图。映射算法基于仿射变换。对正常和LASIK眼以及兔眼进行了基底下神经丛的显微镜检查。基底皮下神经丛的实时成像是大规模进行的,最大尺寸为3.2mm x 3.2mm。所开发的方法实现了对基底下神经丛的实时体内映射,这对于有关形态计量神经丛改变的统计学上可靠的结论是非常必要的。

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